A common precursor for hematopoietic and endothelial cells
| Autor: | John C. Papadimitriou, Alexander Kazarov, Kyunghee Choi, Marion Kennedy, Gordon Keller |
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| Předmět: |
Genetic Markers
Vascular Endothelial Growth Factor A Population Embryoid body Endothelial Growth Factors Biology Polymerase Chain Reaction Colony-Forming Units Assay Mice Precursor cell Aorta-gonad-mesonephros Cell Adhesion Animals Receptors Growth Factor education Molecular Biology Cells Cultured DNA Primers Hemogenic endothelium education.field_of_study Lymphokines Vascular Endothelial Growth Factors Stem Cells Gene Expression Regulation Developmental Receptor Protein-Tyrosine Kinases Cell Differentiation Hematopoietic Stem Cells Embryonic stem cell Cell biology Hematopoiesis Vascular endothelial growth factor A Microscopy Electron Receptors Vascular Endothelial Growth Factor Immunology Hemangioblast Endothelium Vascular Developmental Biology |
| Zdroj: | Scopus-Elsevier |
| Popis: | Embryonic stem cell-derived embryoid bodies contain a unique precursor population which, in response to vascular endothelial growth factor, gives rise to blast colonies in semi-solid medium. Upon transfer to liquid culture with appropriate cytokines, these blast colonies generate both hematopoietic and adherent, stromal-type cells. Cells within the adherent population display characteristics of endothelial lineage including the expression of CD31, flk-1, flt-1, tie-2, the capacity to take up acetylated LDL and the presence of cytoplasmic Weibel-Palade bodies. Mixing studies demonstrated that the hematopoietic and endothelial precursors within the blast colonies develop from the same cell, the blast colony-forming cell. Kinetic analysis showed that the blast colony-forming cell represents a transient cell population that develops early and is lost quickly during embryoid body development. These findings provide strong evidence that the blast colony-forming cell represents the long-hypothesized hemangioblast, the common precursor of the hematopoietic and endothelial lineages. |
| Databáze: | OpenAIRE |
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