Rapid qualitative and quantitative analyses of anthocyanin composition in berries from the Tibetan Plateau with UPLC-quadruple-Orbitrap MS and their antioxidant activities
| Autor: | Yiqun Zhou, Hongyang Zhang, Qingni Sang, Qiuxia Dong, Yuxi Li, Qiangqiang Jia, Ping Hu, Tingting Xiao, Shoude Zhang, Mingfang Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Lycium ruthenicum 030309 nutrition & dietetics medicine.medical_treatment Rosa Tibet High-performance liquid chromatography Antioxidants Mass Spectrometry Anthocyanins 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Hippophae medicine Chromatography High Pressure Liquid Spectroscopy 0303 health sciences geography Plants Medicinal Plateau geography.geographical_feature_category Traditional medicine biology Plant Extracts Chemistry Orbitrap ms Hippophae rhamnoides 04 agricultural and veterinary sciences General Medicine Lycium biology.organism_classification 040401 food science Atomic and Molecular Physics and Optics Fruit Anthocyanin Composition (visual arts) Drugs Chinese Herbal |
| Zdroj: | European Journal of Mass Spectrometry. 26:301-308 |
| ISSN: | 1751-6838 1469-0667 |
| DOI: | 10.1177/1469066720926435 |
| Popis: | Nitraria tangutorum B. (NT), Hippophae rhamnoides L. (HR), Lycium ruthenicum M. (LR), Lycii fructus (LF), Rosa xanthina L. (RX), and Rubuscor chorifolius L. f. (RC) are six berries from Tibetan Plateau. They have been used in traditional folk medicine with a long history, which are rich in anthocyanins. However, detailed study of their anthocyanins remains scarce. Therefore, a method for rapid simultaneous identification and quantification of 12 anthocyanins from berries using UPLC-Quadruple-Orbitrap MS system (UPLC-Q-Orbitrap MS) was established in this work. It was verified with limit of detection (3.86–11.61 µg/L), limit of quantification (3.86–11.61 µg/L), precision (0.95–2.38%), repeatability (0.96–2.08%), stability (0.86–2.31%), mean recovery (95.8–103.1%), recovery range (93.1–107.2%) and RSD less than 5.21%. It was then used in the analysis of anthocyanins in six berries species; 8, 7, 7, 7, 6 and 9 species of anthocyanins have been identified in NT, LF, LR, HR, RC and RX, respectively based on their own retention time and exact mass in positive mode, and for the first time quantified successfully in each berry (31.11 ± 0.42–2978 ± 25.67 μg.g−1). Finally, 2, 2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity (0.92 ± 0.12–5.61 ± 0.23 mM TE/100 g), ferric reducing antioxidant power (FRAP) (1.23 ± 0.15–7.42 ± 0.28 mM TE/100 g) and total antioxidant activity (T-AOC) assays were used to evaluate the antioxidant activities of the six berries. |
| Databáze: | OpenAIRE |
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