FgPEX1 and FgPEX10 are required for the maintenance of Woronin bodies and full virulence of Fusarium graminearum
| Autor: | Shaohua Sun, Hansong Dong, Jinfeng Yu, Yuancun Liang, Chunjie Liu, Lina Wang, Aixin Liu, Li Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Mutant Virulence Conidiation Biology Fungal Proteins Peroxins 03 medical and health sciences Fusarium Microscopy Electron Transmission Woronin body Acetyl Coenzyme A Cell Wall Lipid droplet Organelle Peroxisomes Genetics Plant Diseases 030304 developmental biology 0303 health sciences fungi 030302 biochemistry & molecular biology Membrane Proteins food and beverages Lipid Droplets General Medicine Spores Fungal Peroxisome Yeast Cell biology ATPases Associated with Diverse Cellular Activities Reactive Oxygen Species Trichothecenes |
| Zdroj: | Current Genetics. 65:1383-1396 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/s00294-019-00994-8 |
| Popis: | Peroxisomes are ubiquitous single-membrane-bound organelles that perform a variety of biochemical functions in eukaryotic cells. Proteins involved in peroxisomal biogenesis are collectively called peroxins. Currently, functions of most peroxins in phytopathogenic fungi are poorly understood. Here, we report identification of PEX1 and PEX10 in the phytopathogenic fungus, Fusarium graminearum, namely FgPEX1 and FgPEX10, the orthologs of yeast ScPEX1 and ScPEX10. To functionally characterize FgPEX1 and FgPEX10, we constructed deletion mutants of FgPEX1 and FgPEX10 (ΔPEX1 and ΔPEX10) by targeting gene-replacement strategies. Our data demonstrate that both mutants displayed reduced mycelial growth, conidiation, and production of perithecia. Deletion of FgPEX1 and FgPEX10 resulted in a shortage of acetyl-CoA, which is an important reason for the reduced deoxynivalenol production and inhibited virulence of F. graminearum. Moreover, ΔPEX1 and ΔPEX10 showed an increased accumulation of lipid droplets and endogenous reactive oxygen species. In addition, FgPEX1 and FgPEX10 were found to be involved in the maintenance of cell wall integrity and Woronin bodies. |
| Databáze: | OpenAIRE |
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