Understanding the Relationship between Intrinsic Cardiorespiratory Fitness and Serum and Skeletal Muscle Metabolomics Profile
| Autor: | Marina Lívia Venturini Ferreira, Alex Castro, Celene Fernandes Bernardes, Renata Garbellini Duft, André L. L Andrade, Lucas M. Silva, Mara Patrícia Traina Chacon-Mikahil, Cláudia Regina Cavaglieri |
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| Rok vydání: | 2021 |
| Předmět: |
Serum
0301 basic medicine Muscle tissue medicine.medical_specialty Magnetic Resonance Spectroscopy Vastus lateralis muscle Biochemistry Article Young Adult 03 medical and health sciences chemistry.chemical_compound Blood serum Internal medicine medicine Humans Metabolomics Muscle Skeletal 030102 biochemistry & molecular biology biomarkers Skeletal muscle Cardiorespiratory fitness General Chemistry Ornithine Glutamine 030104 developmental biology medicine.anatomical_structure Endocrinology Cardiorespiratory Fitness chemistry intrinsic cardiorespiratory fitness Blood drawing |
| Zdroj: | Journal of Proteome Research |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/acs.jproteome.0c00905 |
| Popis: | Intrinsic cardiorespiratory fitness (iCRF) indicates the CRF level in the sedentary state. However, even among sedentary individuals, a wide interindividual variability is observed in the iCRF levels, whose associated molecular characteristics are little understood. This study aimed to investigate whether serum and skeletal muscle metabolomics profiles are associated with iCRF, measured by maximal power output (MPO). Seventy sedentary young adults were submitted to venous blood sampling, a biopsy of the vastus lateralis muscle and iCRF assessment. Blood serum and muscle tissue samples were analyzed by proton nuclear magnetic resonance (1H NMR) spectroscopy. Metabolites related to iCRF were those supported by three levels of evidence: (1) correlation with iCRF, (2) significant difference between individuals with low and high iCRF, and (3) metabolite contribution to significant pathways associated with iCRF. From 43 serum and 70 skeletal muscle analyzed metabolites, iCRF was positively associated with levels of betaine, threonine, proline, ornithine, and glutamine in serum and lactate, fumarate, NADP+, and formate in skeletal muscle. Serum betaine and ornithine and skeletal muscle lactate metabolites explained 31.2 and 16.8%, respectively, of the iCRF variability in addition to body mass. The results suggest that iCRF in young adults is positively associated with serum and skeletal muscle metabolic levels, indicative of the amino acid and carbohydrate metabolism. |
| Databáze: | OpenAIRE |
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