Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin
| Autor: | Silvia Giordani, Stefania Fulle, Adriano Piattelli, Michele Baldrighi, Valeria Ettorre, Giovanna Iezzi, Margherita Tumedei, Ester Sara Di Filippo, Rosa Mancinelli, Antonella Fontana, Mariangela Marrone |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biocompatibility
melatonin 02 engineering and technology lcsh:Technology osteogenesis lcsh:Chemistry Melatonin 03 medical and health sciences 0302 clinical medicine bone regeneration Dental pulp stem cells medicine General Materials Science Cell adhesion Bone regeneration hDPSC lcsh:QH301-705.5 Instrumentation Fluid Flow and Transfer Processes biology lcsh:T Chemistry graphene-oxide Process Chemistry and Technology General Engineering 030206 dentistry 021001 nanoscience & nanotechnology lcsh:QC1-999 Computer Science Applications Cell biology RUNX2 lcsh:Biology (General) lcsh:QD1-999 equine bone block lcsh:TA1-2040 Osteocalcin biology.protein Stem cell lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:Physics medicine.drug |
| Zdroj: | Applied Sciences Volume 11 Issue 7 Applied Sciences, Vol 11, Iss 3218, p 3218 (2021) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app11073218 |
| Popis: | Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone blocks were coated with ammonia-functionalized graphene-oxide (G-N) at two different concentrations (2 μg/mL, G-N2 and 10 μg/mL, G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us to quantify the amount of G-N deposited on the blocks. The aim of this study was to investigate in vitro the effect of G-N-coated collagenated equine bone blocks on the proliferation and differentiation of hDPSCs with the addition of a melatonin. This evaluation was determined after 7, 14, and 21 days of culture by the expression of specific microRNAs, RUNX2 and SMAD5 gene expression, osteocalcin levels, and histological analysis. The results showed that equine blocks G-N2 and G-N10 and melatonin gave an optimal cell adhesion as shown by histological analysis, and an increase in the hDPSCs osteogenic potential as confirmed by microRNA and gene expression with an increase in osteocalcin levels. This study suggests that equine bone blocks coated with G-N2 and G-N10 and melatonin promote the osteogenic process. |
| Databáze: | OpenAIRE |
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