In vivo and in vitro effects of microRNA-124 on human gastric cancer by targeting JAG1 through the Notch signaling pathway
| Autor: | Hai-Juan Xiao, Jun-Ming Hou, Lin Yang, Qing Ji, Ren-Ting Li, Cheng Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male JAG1 Cell HES5 Notch signaling pathway Apoptosis Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Cell Movement Stomach Neoplasms medicine Humans Neoplasm Invasiveness HES1 Molecular Biology Aged Cell Proliferation Receptors Notch Cell growth Cell Biology Cell cycle Middle Aged Cell biology Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Female Jagged-1 Protein Signal Transduction |
| Zdroj: | Journal of cellular biochemistry. 119(3) |
| ISSN: | 1097-4644 |
| Popis: | In this study, we aim to determine the function of miR-124 on gastric cancer (GC) cells and the underlying mechanism that involves jaddeg1 (JAG1) and the Notch signaling pathway. GC tissues and adjacent tissues from 100 patients suffering from GC were selected. GC SGC-7901 and AGS cells were selected and grouped into control, mimic-NC, miR-124 mimic, inhibitor-NC, miR-124 inhibitor, and miR-124 inhibitor + si-JAG1 groups. RT-qPCR and a western blotting assay were conducted to detect the expression of miR-124, JAG1 and Notch signaling pathway-related proteins (NICD, HES1 and HES5). MTS, wound-healing, transwell assay and flow cytometry were performed to detect cell proliferation, migration, invasion, cell cycle distribution and apoptosis, respectively. Compared with adjacent tissues, a lower miR-124 expression and higher JAG1 expression were found in GC tissues. JAG1 is a direct target gene of miR-124. Compared with the control group, the expression of JAG1, NICD, HES1, and HES5, cell invasion, migration and proliferation in the miR-124 mimic group were decreased, while the apoptosis rate was increased and cells were arrested at the G0/G1 phase. Compared with the miR-124 inhibitor group, the expression of JAG1, NICD, HES1, and HES5, cell invasion, migration and proliferation in the miR-124 inhibitor + si-JAG1 group were decreased, while the apoptosis rate and cell ratio at the G0/G1 phase were increased. The demonstration that miR-124 inhibits GC cell growth supports the concept that miR-124 functions as a tumor suppressor by a mechanism that involves translational repression of the JAG1 and the inhibition of Notch signaling pathway. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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