The apple RING-H2 protein MdCIP8 regulates anthocyanin accumulation and hypocotyl elongation by interacting with MdCOP1
| Autor: | Chun-Xiang You, Lu-Lu Fu, Yu-Jin Hao, Xiao-Fei Wang, Ting-Ting Zhang, Hui Kang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Light Ubiquitin-Protein Ligases Mutant Plant Science 01 natural sciences Homology (biology) Hypocotyl Anthocyanins 03 medical and health sciences Gene Expression Regulation Plant Arabidopsis Genetics Plant Proteins biology fungi General Medicine biology.organism_classification Phenotype Yeast Cell biology 030104 developmental biology Malus Mutation Photomorphogenesis Elongation Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant science : an international journal of experimental plant biology. 301 |
| ISSN: | 1873-2259 |
| Popis: | COP1, an important RING ubiquitin ligase E3, is a molecular switch for light regulation in plant development. As an interacting protein of COP1, CIP8 contains a RING-H2 domain, but its biological function is unclear. Here, the apple MdCIP8 was identified based on its homology with AtCIP8 in Arabidopsis. MdCIP8 was constitutively expressed at different levels in various apple tissues, and the expression level of MdCIP8 was not affected by light and dark conditions. MdCIP8 reversed the short hypocotyl phenotype of the cip8 mutant under light conditions. Furthermore, the yeast two-hybrid experiment showed that MdCIP8 interacted with the RING domain of MdCOP1 through its RING-H2 domain. MdCIP8-OX/cop1-4 exhibited the phenotype of the cop1-4 mutant, indicating that CIP8 acts upstream of COP1. In addition, an apple transient injection experiment showed that MdCIP8 inhibited anthocyanin accumulation in an MdCOP1-dependent pathway. Overall, our findings reveal that CIP8 plays an inhibitory role in the light-regulation responses of plants. |
| Databáze: | OpenAIRE |
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