Cell mediated immune response elicited in mice after immunization with the P64k meningococcal protein: epitope mapping
| Autor: | Sonia González, Kanury V.S. Rao, Consuelo Nazábal, Osvaldo Reyes, Hilda Garay, Gretel Sardiñas, Evelin Caballero, Ricardo Silva, Julio C. Alvarez-Obregón |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Immunology Molecular Sequence Data Epitopes T-Lymphocyte Meningococcal Vaccines Biology Neisseria meningitidis Lymphocyte Activation Microbiology Epitope Mice Immune system Antigen medicine Immunology and Allergy Animals Amino Acid Sequence Interleukin 4 Mice Inbred BALB C General Medicine T helper cell T lymphocyte Th1 Cells Recombinant Proteins Meningococcal Infections Infectious Diseases medicine.anatomical_structure Epitope mapping Interleukin 12 Female Immunization Lymph Nodes Peptides Epitope Mapping Bacterial Outer Membrane Proteins |
| Zdroj: | FEMS immunology and medical microbiology. 42(2) |
| ISSN: | 0928-8244 |
| Popis: | The P64k protein of Neisseria meningitidis has been reported as an immunological carrier for weak immunogens. This investigation was aimed at characterizing the T-cell response produced in primed mice and at identifying T helper cell epitopes within this molecule. BALB/c mice subcutaneously immunized with the recombinant antigen provided inguinal lymph node cells (LNC) that proliferated in the presence of P64k in a dose-dependent manner. Proliferating cells secreted IL-4 while the concentration of IL-12 remained unaltered in the culture supernatant. By testing a panel of 59 overlapping synthetic peptides spanning the entire sequence of the antigen a T-cell determinant was localized. Prime-boost and lymphoproliferation experiments, conducted with highly purified synthetic peptides, confirmed that the segment including amino acids 470–485 comprises a T-cell epitope within the P64k molecule. |
| Databáze: | OpenAIRE |
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