Exopolysaccharide production by nitrogen-fixing bacteria within nodules of Medicago plants exposed to chronic radiation in the Chernobyl exclusion zone
| Autor: | Michelle Pillon, Jean-Claude Laberche, Anne Le Flèche-Matéos, Bernard Courtois, David Lesur, Nathalie Pawlicki-Jullian, Nadia Goncharova, Josiane Courtois |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Magnetic Resonance Spectroscopy Republic of Belarus Molecular Sequence Data Mannose Microbiology Fucose chemistry.chemical_compound Enterobacteriaceae Glucuronic Acid Medicago Monosaccharide Molecular Biology chemistry.chemical_classification Sinorhizobium meliloti biology Polysaccharides Bacterial Galactose Klebsiella oxytoca DNA-Directed RNA Polymerases Sequence Analysis DNA General Medicine biology.organism_classification Raoultella terrigena Molecular Weight Glucose Chernobyl Nuclear Accident chemistry Root Nodules Plant Bacteria |
| Zdroj: | Research in Microbiology. 161:101-108 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/j.resmic.2009.12.009 |
| Popis: | Nitrogen-fixing bacteria isolated from root nodules of Medicago plants growing in the 10 km zone around the Chernobyl nuclear power plant were screened for the production of new water-soluble acidic exopolysaccharides (EPSs). The different strains belonged to the Enteriobacteriaceae family (Enterobacter ludwigii, Raoultella terrigena, Klebsiella oxytoca), except for one which belonged to the Rhizobiaceae family (Sinorhizobium meliloti). All of the bacteria produced highly viscous EPS with an average molecular weight comprised between 1 x 10(6) and 3 x 10(6) Da. Five different compositions of EPS were characterized by physico-chemical analyses and (1)H NMR spectroscopy: galactose/mannose (2/1), galactose/glucose (1/1), galactose/glucose/mannose (1/2/1), fucose/galactose/glucose (2/1/1) and fucose/galactose/glucose/mannose (2/2/1/1 or 1/1/2/4). Glucuronic acid, a charged monosaccharide, was also recovered in most of the different EPSs. |
| Databáze: | OpenAIRE |
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