Influence of threonine exporters on threonine production in Escherichia coli
| Autor: | Lothar Eggeling, Reinhard Krämer, Daniela Kruse, Y. J. Chung, Milton H. Saier, Andreas Burkovski, M. Rieping, Walter Pfefferle, Jason Tchieu |
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| Rok vydání: | 2002 |
| Předmět: |
Threonine
Escherichia coli Proteins Membrane Proteins Heterologous Biological Transport General Medicine Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Enterobacteriaceae Corynebacterium glutamicum Amino Acid Transport Systems Neutral Bacterial Proteins Biochemistry Escherichia coli medicine Efflux Carrier Proteins Gene Bacteria Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 59:205-210 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-002-0987-7 |
| Popis: | Threonine production in Escherichia coli threonine producer strains is enhanced by overexpression of the E. coli rhtB and rhtC genes or by heterologous overexpression of the gene encoding the Corynebacterium glutamicum threonine excretion carrier, thrE. Both E. coli genes give rise to a threonine-resistant phenotype when overexpressed, and they decrease the accumulation of radioactive metabolites derived from [(14)C] L-threonine. The evidence presented supports the conclusion that both RhtB and RhtC catalyze efflux of L-threonine and other structurally related neutral amino acids, but that the specificities of these two carriers differ substantially. |
| Databáze: | OpenAIRE |
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