Molecular evolution of the haemagglutinin–neuraminidase gene in human parainfluenza virus type 3 isolates from children with acute respiratory illness in Yamagata prefecture, Japan
| Autor: | Tatsuya Ikeda, Tsutomu Itagaki, Hiroyuki Tsukagoshi, Chieko Abiko, Hirokazu Kimura, Yoko Aoki, Manami Nagano, Masahiro Noda, Katsumi Mizuta |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Microbiology (medical) Adolescent Sequence analysis Molecular Sequence Data Neuraminidase Biology Respirovirus Infections Microbiology Virus Evolution Molecular Japan Mutation Rate Molecular evolution Genetic variation Cluster Analysis Humans HN Protein Child Respiratory Tract Infections Gene Phylogeny Phylogenetic tree Infant Newborn Genetic Variation Infant Sequence Analysis DNA General Medicine Virology Parainfluenza Virus 3 Human Human Parainfluenza Virus Child Preschool Female |
| Zdroj: | Journal of Medical Microbiology. 63:570-577 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | We conducted detailed genetic analyses of the haemagglutinin–neuraminidase (HN) gene in 272 human parainfluenza virus type 3 (HPIV3) isolates from children with acute respiratory illness during the period 2002–2009 in Yamagata prefecture, Japan. A phylogenetic tree reconstructed by the Bayesian Markov chain Monte Carlo method showed that the strains diversified at around 1946 and that the rate of molecular evolution was 1.10×10−3 substitutions per site per year. Identity was high among the present strains (pairwise-distances were short. Furthermore, we found four positive selection sites and some key amino acid substitutions in active/catalytic sites of the HN protein. The results suggest that the HN gene of HPIV3 in the present strains evolved rapidly, similarly to other virus genes such as the G gene of respiratory syncytial virus. However, the biological functions and detailed structures of the HN glycoprotein in some of these strains may have been altered. |
| Databáze: | OpenAIRE |
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