Impact of formate supplementation on body weight and plasma amino acids
| Autor: | Jacqueline Tait-Mulder, Seth B. Coffelt, Matthias Pietzke, Dimitris Athineos, Robert Wiesheu, Alexei Vazquez, Sandeep Dhayade, Karen Blyth, David Sumpton |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Formates microbiome lcsh:TX341-641 Spleen Article Mice 03 medical and health sciences chemistry.chemical_compound Immune system formate Internal medicine Blood plasma medicine Animals Formate Amino Acids Phylogeny chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics Hematology business.industry Chemistry Sodium formate Body Weight Carbohydrate metabolomics Gastrointestinal Microbiome Amino acid immune system 030104 developmental biology Endocrinology medicine.anatomical_structure Sample Size Dietary Supplements Female Bifidobacterium business lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Nutrients, Vol 12, Iss 2181, p 2181 (2020) Volume 12 Issue 8 |
| ISSN: | 2072-6643 |
| Popis: | Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM of sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology analyser. At the end of the study, blood and tissues were collected for metabolomics analysis and immune cell profiling. Formate supplementation had no significant physiological effect on male mice, except for a small decrease in body weight. Formate supplementation had no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice, however, the body weight and spleen wet weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of bifidobacteria, a probiotic bacterium, in the stools of female mice. We conclude that formate supplementation induces physiological changes in a gender-specific manner. |
| Databáze: | OpenAIRE |
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