Deep tissue scattering compensation with three-photon F-SHARP
| Autor: | Mathias Babot, Caroline Berlage, Benjamin Judkewitz, Matthew E. Larkum, Malinda L. S. Tantirigama, Clarissa J. Whitmire, Diego Di Battista, James F.A. Poulet, Ioannis N. Papadopoulos |
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| Rok vydání: | 2021 |
| Předmět: |
Wavefront
Physics 0303 health sciences Photon Scattering business.industry 01 natural sciences Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Compensation (engineering) 010309 optics 03 medical and health sciences Optics Deep tissue 0103 physical sciences Medical imaging business Penetration depth Excitation 030304 developmental biology |
| Zdroj: | Optica |
| ISSN: | 2334-2536 |
| DOI: | 10.1364/optica.440279 |
| Popis: | Optical imaging techniques are widely used in biological research, but their penetration depth is limited by tissue scattering. Wavefront shaping techniques are able to overcome this problem in principle, but are often slow, and their performance depends on the sample. This greatly reduces their practicability for biological applications. Here we present a scattering compensation technique based on three-photon excitation, which converges faster than comparable two-photon (2P) techniques and works reliably even on densely labeled samples, where 2P approaches fail. To demonstrate its usability and advantages for biomedical imaging, we apply it to the imaging of dendritic spines on layer 5 neurons labeled with green fluorescent protein in an anesthetized mouse. |
| Databáze: | OpenAIRE |
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