A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission
| Autor: | Hyoung F. Kim, Chuljung Kwak, Seo-Hee Ahn, Sun Lim Choi, Juyoun Yoo, Somi Kim, Deok-Jin Jang, Yong-Seok Lee, Soo Young Choi, Dae Won Kim, Jun Hyeok Choi, Chulhun Kang, Nam Kyung Yu, Chae Seok Lim, Jaehoon Shim, Su Eon Sim, Bong-Kiun Kaang |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
CCCTC-Binding Factor Cell Membrane Permeability Neurogenesis Biology Hippocampus Synaptic Transmission Article 03 medical and health sciences Gene expression Animals Humans Nuclear protein RNA Small Interfering Cells Cultured Neurons Gene knockdown Multidisciplinary HEK 293 cells Nuclear Proteins Dendrites Cell biology Mice Inbred C57BL Repressor Proteins 030104 developmental biology HEK293 Cells CTCF Signal transduction Neural development Signal Transduction |
| Zdroj: | Scientific Reports SCIENTIFIC REPORTS(6) |
| ISSN: | 2045-2322 |
| Popis: | Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development. |
| Databáze: | OpenAIRE |
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