Protein Kinase D regulates several aspects of development in Drosophila melanogaster
| Autor: | Angelika Hausser, Dieter Maier, Sabrina J. Kugler, Anja C. Nagel, Helena Gloc, Anette Preiss, Irmgard Wech |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Aging
Light Recombinant Fusion Proteins Cell Line Inhibitor of Apoptosis Proteins Animals Genetically Modified RNA interference Animals Drosophila Proteins Humans Wings Animal lcsh:QH301-705.5 Protein kinase C Protein Kinase C biology Kinase fungi Wild type biology.organism_classification musculoskeletal system Molecular biology Phenotype Cell biology Isoenzymes Drosophila melanogaster lcsh:Biology (General) Larva cardiovascular system Photoreceptor Cells Invertebrate RNA Interference Signal transduction Drosophila Protein Developmental Biology Research Article Signal Transduction |
| Zdroj: | BMC Developmental Biology BMC Developmental Biology, Vol 7, Iss 1, p 74 (2007) |
| ISSN: | 1471-213X |
| Popis: | Background Protein Kinase D (PKD) is an effector of diacylglycerol-regulated signaling pathways. Three isoforms are known in mammals that have been linked to diverse cellular functions including regulation of cell proliferation, differentiation, motility and secretory transport from the trans-Golgi network to the plasma membrane. In Drosophila, there is a single PKD orthologue, whose broad expression implicates a more general role in development. Results We have employed tissue specific overexpression of various PKD variants as well as tissue specific RNAi, in order to investigate the function of the PKD gene in Drosophila. Apart from a wild type (WT), a kinase dead (kd) and constitutively active (SE) Drosophila PKD variant, we also analyzed two human isoforms hPKD2 and hPKD3 for their capacity to substitute PKD activity in the fly. Overexpression of either WT or kd-PKD variants affected primarily wing vein development. However, overexpression of SE-PKD and PKD RNAi was deleterious. We observed tissue loss, wing defects and degeneration of the retina. The latter phenotype conforms to a role of PKD in the regulation of cytoskeletal dynamics. Strongest phenotypes were larval to pupal lethality. RNAi induced phenotypes could be rescued by a concurrent overexpression of Drosophila wild type PKD or either human isoform hPKD2 and hPKD3. Conclusion Our data confirm the hypothesis that Drosophila PKD is a multifunctional kinase involved in diverse processes such as regulation of the cytoskeleton, cell proliferation and death as well as differentiation of various fly tissues. |
| Databáze: | OpenAIRE |
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