Interleukin-6 is a positive regulator of tumor necrosis factor α-induced adipose-related protein in 3T3-L1 adipocytes

Autor: Matthias Blüher, Mathias Fasshauer, Anne-Marie Chambaut-Guérin, Johannes Klein, Ralf Paschke, Ulrike Lossner, Margit Klier, Susan Kralisch
Rok vydání: 2004
Předmět:
medicine.medical_specialty
Time Factors
medicine.medical_treatment
Biophysics
Adipose tissue
Biochemistry
Mice
chemistry.chemical_compound
Downregulation and upregulation
Structural Biology
3T3-L1 Cells
Proto-Oncogene Proteins
Internal medicine
Adipocyte
Adipocytes
Genetics
medicine
Animals
Tumor necrosis factor α
RNA
Messenger
Obesity
Enzyme Inhibitors
Interleukin 6
Molecular Biology
Dose-Response Relationship
Drug
biology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Interleukin-6
Tumor necrosis factor α-induced adipose-related protein
Membrane Proteins
Interleukin
Insulin resistance
3T3-L1
Cell Biology
Janus Kinase 2
Protein-Tyrosine Kinases
Tyrphostins
Stimulation
Chemical
Endocrinology
Cytokine
Gene Expression Regulation
chemistry
biology.protein
Tumor necrosis factor alpha
Zdroj: FEBS Letters. 560:153-157
ISSN: 0014-5793
Popis: Tumor necrosis factor (TNF) alpha-induced adipose-related protein (TIARP) is a novel TNFalpha-stimulated protein in adipocytes. Besides TNFalpha, interleukin (IL)-6 has recently been shown to be another adipocytokine implicated in insulin resistance. Therefore, the impact of IL-6 on TIARP gene expression in 3T3-L1 adipocytes was determined by quantitative real-time reverse transcription-polymerase chain reaction. Interestingly, TIARP mRNA expression was stimulated up to 3.8-fold by IL-6 in a dose-dependent fashion with significant stimulation detectable at effector concentrations as low as 3 ng/ml and maximal effects seen at 100 ng/ml IL-6. Induction of TIARP mRNA by IL-6 was time-dependent with significant upregulation occurring as early as 2 h after effector addition and maximal effects observed at 4 h. In parallel, TIARP protein synthesis was upregulated with maximal effects seen after 8 h of IL-6 treatment. Furthermore, the Janus kinase 2 inhibitor AG490 decreased TIARP mRNA expression. The increase of TIARP mRNA could be reversed by withdrawal of IL-6 for 24 h. Furthermore, TIARP mRNA induction by IL-6 was also seen in brown adipocytes but not in muscle and liver cells. Taken together, these results show that TIARP is acutely regulated in adipose tissue not only by TNFalpha but also by IL-6 which has been shown to be another important cytokine implicated in the pathogenesis of insulin resistance.
Databáze: OpenAIRE
Externí odkaz: