Biochips for Direct Detection and Identification of Bacteria in Blood Culture-Like Conditions
| Autor: | Thierry Livache, Sandrine Boisset, S. Slimani, Raphael Mathey, Vincent Templier, Yoann Roupioz, M. Maurin |
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| Přispěvatelé: | Chimie pour la Reconnaissance et l’Etude d’Assemblages Biologiques (CREAB), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire Grenoble Alpes (CHU Grenoble Alpes), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre Hospitalier Universitaire [Grenoble] (CHU) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors [SDV.BIO]Life Sciences [q-bio]/Biotechnology Antibody microarray 030106 microbiology Protein Array Analysis lcsh:Medicine Bacteremia Bacterial growth Article Microbiology 03 medical and health sciences medicine Humans Serologic Tests Blood culture lcsh:Science ComputingMilieux_MISCELLANEOUS Hematologic Tests Multidisciplinary biology medicine.diagnostic_test Diagnostic Tests Routine lcsh:R Gold standard (test) Bacteria Present Surface Plasmon Resonance biology.organism_classification medicine.disease Antibodies Bacterial 3. Good health Early Diagnosis 030104 developmental biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Salmonella enteritidis Blood Culture Salmonella Infections Feasibility Studies lcsh:Q Bacteria Blood sampling |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2017, 7 (1), ⟨10.1038/s41598-017-10072-z⟩ Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) Scientific Reports, 2017, 7 (1), ⟨10.1038/s41598-017-10072-z⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-10072-z⟩ |
| Popis: | Bloodstream bacterial infections are life-threatening conditions necessitating prompt medical care. Rapid pathogen identification is essential for early setting of the best anti-infectious therapy. However, the bacterial load in blood samples from patients with bacteremia is too low and under the limit of detection of most methods for direct identification of bacteria. Therefore, a preliminary step enabling the bacterial multiplication is required. To do so, blood cultures still remain the gold standard before bacteremia diagnosis. Bacterial identification is then usually obtained within 24 to 48 hours -at least- after blood sampling. In the present work, the fast and direct identification of bacteria present in blood cultures is completed in less than 12 hours, during bacterial growth, using an antibody microarray coupled to a Surface Plasmon Resonance imager (SPRi). Less than one bacterium (Salmonella enterica serovar Enteritidis) per milliliter of blood sample is successfully detected and identified in blood volumes similar to blood tests collected in clinics (i.e. several milliliters). This proof of concept demonstrates the workability of our method for human samples, despite the highly complex intrinsic nature of unprocessed blood. Our label-free method then opens new perspectives for direct and faster bacterial identification in a larger range of clinical samples. |
| Databáze: | OpenAIRE |
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