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It is known that introducing a plasmid into bacteria leads to metabolic burden and host gene expression alteration, and that introduction of ColE1-type plasmid into bacteria also changes global metabolic pathways. However, the underlying mechanisms coordinating these changes are still unclear. Since ColE1-type plasmids are widely used in many biological fields, understanding the mechanism underlying the effects of the ColE1 plasmid on host gene expression is critical. In our study, we focus on the plasmid-encoded noncoding RNA that normally negatively regulates plasmid copy number. We found that ectopic expression of plasmid-encoded noncoding RNA on control plasmid can reverse the host gene expression changed by introduction of the control plasmid itself. Further, using in vitro gel shift assay we found that the plasmid-encoded noncoding RNA can bind to host factor Hfq. Furthermore, deletion of Hfq decreases the expression level of plasmid-encoded noncoding RNA. Together, we propose that ectopically expressed, plasmid-encoded noncoding RNA may compete with host RNA species for Hfq binding, which further disrupts host gene expression normally responsive to introduction of plasmid itself. |
