Molecular packing parameters of bipolar lipids
| Autor: | Agata Gambacorta, Annalisa Relini, Z. Mirghani, D. Bertoia, A. Gliozzi, F. Cavagnetto |
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| Rok vydání: | 1992 |
| Předmět: |
Chromatography
Hot Temperature Ionophores Chemistry ved/biology Vesicle Sulfolobus solfataricus ved/biology.organism_classification_rank.species Biophysics Membrane structure Molecular Conformation Cell Biology Models Theoretical Biochemistry Lipids Light scattering Sulfolobus Molecular geometry X-Ray Diffraction Chemical physics Molecule Self-assembly Ion transporter Mathematics Phospholipids |
| Zdroj: | Biochimica et biophysica acta. 1106(2) |
| ISSN: | 0006-3002 |
| Popis: | The bipolar lipid fractions extracted from the thermophilic archaeobacterium Sulfolobus solfataricus have different chemical structures and geometrical shapes. The conditions which lead to the formation of vesicles were investigated in order to study the self-assembly of these molecules. Such conditions are fulfilled when an appropriate mixture of two different molecular species (both bipolar or bipolar and monopolar) is used. According to the theory introduced by Israelachvili and co-workers, lipid self-assembly results from the balance of interaction free energy, entropy and molecular geometry. We have shown that this theory can be extended to bipolar lipids, in spite of their more complex nature, and the experimental results obtained combining 1H-NMR, light scattering and entrapped volume techniques closely match theoretical expectations. To carry out calculations, it was necessary to introduce hypotheses about the disposition of bipolar molecules in the vesicle membrane. These hypotheses have been tested indirectly by measuring the transport properties mediated by carriers or channels, whose transport mechanism can be considered to be a probe of the membrane structure. |
| Databáze: | OpenAIRE |
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