The heterologous production of terpenes by the thermophile Parageobacillus thermoglucosidasius in a consolidated bioprocess using waste bread
| Autor: | Matthew Q. Styles, Edward A. Nesbitt, Timothy D. Hoffmann, David J. Leak, Junichi Queen, Maria V. Ortenzi |
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| Rok vydání: | 2021 |
| Předmět: |
Isopentenyl pyrophosphate
Mevalonic Acid Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound medicine Bioprocess Bacillaceae Escherichia coli 030304 developmental biology 0303 health sciences biology Terpenes 030306 microbiology Thermophile Bread biology.organism_classification Metabolic pathway Biochemistry chemistry Mevalonate pathway Flux (metabolism) Biotechnology Archaea |
| Zdroj: | Styles, M Q, Nesbitt, E A, Hoffmann, T, Queen, J, Ortenzi, M V & Leak, D 2021, ' The heterologous production of terpenes by the thermophile Parageobacillus thermoglucosidasius in a consolidated bioprocess using waste bread ', Metabolic Engineering, vol. 65, pp. 146-155 . https://doi.org/10.1016/j.ymben.2020.11.005 |
| ISSN: | 1096-7176 |
| DOI: | 10.1016/j.ymben.2020.11.005 |
| Popis: | Parageobacillus thermoglucosidasius is a genetically tractable thermophile that grows rapidly at elevated temperatures, with a doubling time at 65 °C comparable to the shortest doubling times of Escherichia coli. It is capable of using a wide variety of substrates, including carbohydrate oligomers, and has been developed for the industrial production of ethanol. In this study, P. thermoglucosidasius NCIMB11955 has been engineered to produce the sesquiterpene τ-muurolol by introduction of a heterologous mevalonate pathway constructed using genes from several thermophilic archaea together with a recently characterised thermostable τ-muurolol synthase. P. thermoglucosidasius naturally uses the methylerythritol phosphate pathway for production of the terpene precursor, isopentenyl pyrophosphate, while archaea use a version of the mevalonate pathway. By introducing the orthogonal archaeal pathway it was possible to increase the flux through to sesquiterpene biosynthesis. Construction of such a large metabolic pathway created problems with genetic vector introduction and stability, so recombinant plasmids were introduced by conjugation, and a thermostable serine integrase system was developed for integration of large pathways onto the chromosome. Finally, by making the heterologous pathway maltose-inducible we demonstrate that the new strain is capable of using waste bread directly as an autoinduction carbon source for the production of terpenes in a consolidated bioprocess. |
| Databáze: | OpenAIRE |
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