Rare genetic variation at Zea mays crtRB1 increases β-carotene in maize grain
| Autor: | Jiansheng Li, Torbert Rocheford, Debra J. Skinner, Dean DellaPenna, Edward S. Buckler, Xiaohong Yang, Catherine B. Kandianis, Zhiyuan Fu, Ling Bai, Eun Ha Kim, Thomas P. Brutnell, Sharon E. Mitchell, Qing Li, Maria Zaharieva, Jianbing Yan, Natalia Palacios, Marilyn L. Warburton, Carlos Harjes, Maria G. Salas Fernandez, Yang Fu, Raman Babu |
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| Rok vydání: | 2010 |
| Předmět: |
Germplasm
Genetics Polymorphism Genetic Molecular Sequence Data Quantitative Trait Loci Biofortification Gene Expression food and beverages Quantitative trait locus Biology Genes Plant beta Carotene Zea mays Mixed Function Oxygenases Genetic marker Botany Genetic variation Genetic variability Plant breeding Allele Edible Grain |
| Zdroj: | Nature Genetics. 42:322-327 |
| ISSN: | 1546-1718 1061-4036 |
| Popis: | Breeding to increase beta-carotene levels in cereal grains, termed provitamin A biofortification, is an economical approach to address dietary vitamin A deficiency in the developing world. Experimental evidence from association and linkage populations in maize (Zea mays L.) demonstrate that the gene encoding beta-carotene hydroxylase 1 (crtRB1) underlies a principal quantitative trait locus associated with beta-carotene concentration and conversion in maize kernels. crtRB1 alleles associated with reduced transcript expression correlate with higher beta-carotene concentrations. Genetic variation at crtRB1 also affects hydroxylation efficiency among encoded allozymes, as observed by resultant carotenoid profiles in recombinant expression assays. The most favorable crtRB1 alleles, rare in frequency and unique to temperate germplasm, are being introgressed via inexpensive PCR marker-assisted selection into tropical maize germplasm adapted to developing countries, where it is most needed for human health. |
| Databáze: | OpenAIRE |
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