Interaction of maize Opaque-2 and the transcriptional co-activators GCN5 and ADA2, in the modulation of transcriptional activity
| Autor: | Jan Willem Borst, Riyaz Bhat, Richard D. Thompson, Marcus Riehl |
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| Přispěvatelé: | Max Planck Institute for Plant Breeding Research (MPIPZ), Wageningen University and Research Centre (WUR), UMR 0102 - Unité de Recherche Génétique et Ecophysiologie des Légumineuses, Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
resonance energy-transfer Transcription Genetic protein-protein interactions Plant Science tumor virus promoter Biochemistry in-vivo 01 natural sciences Transactivation Transcription (biology) Enhancer binding Fluorescence Resonance Energy Transfer GENETIQUE VEGETALE Promoter Regions Genetic direct gene-transfer Glucuronidase Histone Acetyltransferases Plant Proteins 2. Zero hunger acidic activation domains 0303 health sciences Microscopy Confocal Protoplasts histone acetylation General Medicine Plants lifetime imaging microscopy Cell biology DNA-Binding Proteins Protein Binding FLIM Recombinant Fusion Proteins Green Fluorescent Proteins Molecular Sequence Data Biochemie Biology Transfection Protein–protein interaction 03 medical and health sciences Bacterial Proteins Acetyltransferases Plant Cells Genetics Nucleosome [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Gene Transcription factor living plant-cells 030304 developmental biology Binding Sites Promoter gpi-anchored proteins Luminescent Proteins CO-ACTIVATORS Trans-Activators FRET ACCEPTOR PHOTOBLEACHING TRANSCRIPTIONAL ACTIVATION Agronomy and Crop Science Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Molecular Biology Plant Molecular Biology, Springer Verlag (Germany), 2004, 55 (2), pp.239-252. ⟨10.1007/s11103-004-0553-z⟩ Plant Molecular Biology 55 (2004) 2 Plant Molecular Biology, 55(2), 239-252 |
| ISSN: | 0167-4412 |
| DOI: | 10.1007/s11103-004-0553-z⟩ |
| Popis: | International audience; Maize Opaque-2 (ZmO2), a bZip class transcription factor has been shown to activate the transcription of a series of genes expressed in the maturation phase of endosperm development. Activation requires the presence of one or more enhancer binding sites, which confer the propensity for activation by ZmO2 on heterologous promoters and in heterologous plant cell types, such as tobacco mesophyll protoplasts. The region of ZmO2 required for conferring transcriptional activation has been localised to a stretch of acidic residues in the N-terminal portion of the ZmO2 sequence, which is conserved between O2-related bZip factor sequences. Previously we identified the maize homologues of yeast transcriptional co-activators GCN5 and ADA2 that are implicated in nucleosome modification and transcription. In the present study we have shown that transcriptional modulation by ZmO2 involves the intranuclear interaction of ZmO2 with ZmADA2 and ZmGCN5. Forster resonance energy transfer (FRET) based techniques have enabled us to estimate the intracellular site of these intermolecular interactions. As a functional readout of these intranuclear interactions, we used the ZmO2 responsive maize b-32 promoter to drive the beta-glucuronidase (GUS) in the presence and absence of ZmGCN5 and ZmADA2. Our results suggest that the likely recruitment of ZmADA2 and ZmGCN5 modulates the transactivation of b-32 promoter by ZmO2 and that there may be a competition between ZmGCN5 and ZmO2 for binding to the amino-terminal of ZmADA2. The results may be taken as a paradigm for other processes of transcriptional modulation in planta involving acidic activation domains. |
| Databáze: | OpenAIRE |
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