Flow cytometric technique for quantitating cytotoxic response to photodynamic therapy
| Autor: | D. L. Campbell, Barbara G. Campling, J. G. Johnson, Francesca Rossi, Michael E. Fisher, Pottier Rh, J. C. Kennedy |
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| Rok vydání: | 1996 |
| Předmět: |
Lung Neoplasms
medicine.diagnostic_test Cell Survival medicine.medical_treatment Protoporphyrins Photodynamic therapy General Medicine Aminolevulinic Acid Biology Flow Cytometry Biochemistry Molecular biology Flow cytometry Staining Photochemotherapy Cell culture medicine Tumor Cells Cultured Cytotoxic T cell Humans Photosensitizer Physical and Theoretical Chemistry Carcinoma Small Cell Cytotoxicity Cellular Debris |
| Zdroj: | Photochemistry and photobiology. 63(1) |
| ISSN: | 0031-8655 |
| Popis: | A simple flow cytometric technique for rapid measurement of multilog cytotoxic responses to photosensitization of cellular systems is described. This technique is particularly useful for cell lines with a low colony-forming efficiency, for which a nonclonogenic assay is required. The assay separates cell-sized objects from cellular debris by gating on forward scatter versus side scatter, identifies viable cells by positive calcein AM and negative ethidium homodimer-1 staining and measures cell concentration relative to an internal standard of polystyrene beads. Large numbers of cells can be analyzed rapidly. Two patient-derived small cell lung cancer cell lines, NCI-H209 and SV-E, were used to test the technique. Photordiation survival curves of the response of these cell lines to 5-aminolevulinic acid-induced protoprophyrin IX photosensitization correlated with the extent of photosensitizer accumulation. There was good agreement between the results obtained using the tritiated thymidine incorporation assay and the flow cytometric cytotoxicity assay. The technique can be used to measure cytotoxic responses to photosensitization of cell lines regardless of their plating efficiencies. |
| Databáze: | OpenAIRE |
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