Silver Nanoparticles from Oregano Leaves’ Extracts as Antimicrobial Components for Non-Infected Hydrogel Contact Lenses
| Autor: | Panagiotis K. Raptis, C. A. Papachristodoulou, Anastasia Meretoudi, Christina N. Banti, Panagiotis Zoumpoulakis, Sotiris K. Hadjikakou, Thomas Mavromoustakos, Nikolaos Kourkoumelis, Aris Ikiades |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Metal Nanoparticles
02 engineering and technology medicine.disease_cause 01 natural sciences Silver nanoparticle antimicrobial materials lcsh:Chemistry X-Ray Diffraction Staphylococcus epidermidis Origanum lcsh:QH301-705.5 Spectroscopy biology Chemistry Hydrogels General Medicine 021001 nanoscience & nanotechnology Antimicrobial Computer Science Applications Anti-Bacterial Agents Staphylococcus aureus Pseudomonas aeruginosa Thermogravimetry 0210 nano-technology silver nanoparticles Silver Contact Lenses contact lens Microbial Sensitivity Tests 010402 general chemistry Catalysis Article Inorganic Chemistry Minimum inhibitory concentration oregano leaves’ extract medicine Animals Humans Physical and Theoretical Chemistry Molecular Biology Escherichia coli Minimum bactericidal concentration Plant Extracts Organic Chemistry Spectrometry X-Ray Emission biology.organism_classification 0104 chemical sciences Contact lens Plant Leaves lcsh:Biology (General) lcsh:QD1-999 Biofilms Artemia Nuclear chemistry |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 3539, p 3539 (2021) Volume 22 Issue 7 |
| ISSN: | 1422-0067 |
| Popis: | The oregano leaves’ extract (ORLE) was used for the formation of silver nanoparticles (AgNPs(ORLE)). ORLE and AgNPs(ORLE) (2 mg/mL) were dispersed in polymer hydrogels to give the pHEMA@ORLE_2 and pHEMA@AgNPs(ORLE)_2 using hydroxyethyl–methacrylate (HEMA). The materials were characterized by X-ray fluorescence (XRF) spectroscopy, X-ray powder diffraction analysis (XRPD), thermogravimetric differential thermal analysis (TG-DTA), derivative thermogravimetry/differential scanning calorimetry (DTG/DSC), ultraviolet (UV-Vis), and attenuated total reflection mode (ATR-FTIR) spectroscopies in solid state and UV–Vis in solution. The crystallite size value, analyzed with XRPD, was determined at 20 nm. The antimicrobial activity of the materials was investigated against Gram-negative bacterial strains Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). The Gram-positive ones of the genus of Staphylococcus epidermidis (S. epidermidis) and Staphylococcus aureus (S. aureus) are known to be involved in microbial keratitis by the means of inhibitory zone (IZ), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The IZs, which developed upon incubation of P. aeruginosa, E. coli, S. epidermidis, and S. aureus with paper discs soaked in 2 mg/mL of AgNPs(ORLE), were 11.7 ± 0.7, 13.5 ± 1.9, 12.7 ± 1.7, and 14.3 ± 1.7 mm. When the same dose of ORLE was administrated, the IZs were 10.2 ± 0.7, 9.2 ± 0.5, 9.0 ± 0.0, and 9.0 ± 0.0 mm. The percent of bacterial viability when they were incubated over the polymeric hydrogel discs of pHEMA@AgNPs(ORLE)_2 was interestingly low (66.5, 88.3, 77.7, and 59.6%, respectively, against of P. aeruginosa, E. coli, S. epidermidis, and S. aureus) and those of pHEMA@ORLE_2 were 89.3, 88.1, 92.8, and 84.6%, respectively. Consequently, pHEMA@AgNPs(ORLE)_2 could be an efficient candidate toward the development of non-infectious contact lenses. |
| Databáze: | OpenAIRE |
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