Identification of Biomarkers for Defense Response to Plasmopara viticola in a Resistant Grape Variety
| Autor: | Giulia Chitarrini, Evelyn Soini, Samantha Riccadonna, Pietro Franceschi, Luca Zulini, Domenico Masuero, Antonella Vecchione, Marco Stefanini, Gabriele Di Gaspero, Fulvio Mattivi, Urska Vrhovsek |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Resistance Vitis vinifera Bianca Biomarkers Grapevine Hybrid Metabolomics Plant pathogen Plasmopara viticola Plant Science ampelopsin H vaticanol C pallidol ampelopsin D quadrangularin A Z-miyabenol C alpha-viniferin biosynthesis phytoalexin lcsh:Plant culture 01 natural sciences Pallidol 03 medical and health sciences chemistry.chemical_compound Flavonols Settore AGR/13 - CHIMICA AGRARIA Plant defense against herbivory lcsh:SB1-1110 Secondary metabolism quadrangularin A Unsaturated fatty acid Original Research chemistry.chemical_classification biology hybrid biomarkers biology.organism_classification plant pathogen grapevine Ampelopsin 030104 developmental biology chemistry Biochemistry Downy mildew Z-miyabenol C 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 8 (2017) Frontiers in Plant Science |
| DOI: | 10.3389/fpls.2017.01524/full |
| Popis: | Downy mildew (Plasmopara viticola) is one of the most destructive diseases of the cultivated species Vitis vinifera. The use of resistant varieties, originally derived from backcrosses of North American Vitis spp., is a promising solution to reduce disease damage in the vineyards. To shed light on the type and the timing of pathogen-triggered resistance, this work aimed at discovering biomarkers for the defense response in the resistant variety Bianca, using leaf discs after inoculation with a suspension of P. viticola. We investigated primary and secondary metabolism at 12, 24, 48, and 96 h post-inoculation (hpi). We used methods of identification and quantification for lipids (LC-MS/MS), phenols (LC-MS/MS), primary compounds (GC-MS), and semi-quantification for volatile compounds (GC-MS). We were able to identify and quantify or semi-quantify 176 metabolites, among which 53 were modulated in response to pathogen infection. The earliest changes occurred in primary metabolism at 24–48 hpi and involved lipid compounds, specifically unsaturated fatty acid and ceramide; amino acids, in particular proline; and some acids and sugars. At 48 hpi, we also found changes in volatile compounds and accumulation of benzaldehyde, a promoter of salicylic acid-mediated defense. Secondary metabolism was strongly induced only at later stages. The classes of compounds that increased at 96 hpi included phenylpropanoids, flavonols, stilbenes, and stilbenoids. Among stilbenoids we found an accumulation of ampelopsin H + vaticanol C, pallidol, ampelopsin D + quadrangularin A, Z-miyabenol C, and α-viniferin in inoculated samples. Some of these compounds are known as phytoalexins, while others are novel biomarkers for the defense response in Bianca. This work highlighted some important aspects of the host response to P. viticola in a commercial variety under controlled conditions, providing biomarkers for a better understanding of the mechanism of plant defense and a potential application in field studies of resistant varieties. |
| Databáze: | OpenAIRE |
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