Genetic changes and expression of the mannose 6-phosphate/insulin-like growth factor II receptor gene in human hepatitis B virus-associated hepatocellular carcinoma
| Autor: | Er-Bin Yang, Kai Zhang, Liu-Liang Qin, Pierce K. H. Chow, Yin-Nong Zhao |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Carcinoma Hepatocellular DNA Complementary Gene Expression Loss of Heterozygosity Locus (genetics) Mannose 6-phosphate Biology medicine.disease_cause Receptor IGF Type 2 Loss of heterozygosity chemistry.chemical_compound Genetics medicine Humans Deoxyguanosine RNA Messenger RNA Neoplasm Gene Hepatitis B virus Base Sequence Liver Neoplasms Insulin-like growth factor 2 receptor General Medicine Middle Aged Hepatitis B medicine.disease Molecular biology digestive system diseases chemistry Hepatocellular carcinoma Mutation Cancer research Female |
| Zdroj: | International Journal of Molecular Medicine. |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | It was reported that 60-70% of hepatitis B virus (HBV)-negative hepatocellular carcinoma (HCC) had loss of heterozygosity (LOH) at the mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) locus and this gene was mutated in 55% of these patients with LOH. In this study, genomic DNA from 29 pairs of HBV-positive HCC and corresponding non-tumor tissues was used to analyze LOH at the M6P/IGF2R locus and single deoxyguanosine deletion in this gene by PCR. Total RNA from 19 of the 29 patients was utilized to determine a 192 bp insert in the M6P/IGF2R mRNA and expression of this gene by RT-PCR. Twenty-eight of 29 (97%) HBV-positive HCC were found to be informative at the M6P/IGF2R locus but LOH at this region was only detected in 4/28 (14%) informative patients. Neither single deoxyguanosine deletion in this gene nor 192 bp insert in its mRNA occurred in these patients. Compared with corresponding non-tumor tissues, expression of the M6P/IGF2R mRNA was decreased in 13/19 (68%) HBV-positive HCC tissues, suggesting that M6P/IGF2R may be involved in HBV-associated hepato-carcinogenesis by the regulation of its expression level. In the development of HBV-associated HCC, M6P/IGF2R mutation may not be a major agent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|