Determination of nitecapone and (13C6)nitecapone in human plasma by gas chromatography/mass spectrometry
| Autor: | Pekka Ottoila, Carita Karlsson, Jyrki Taskinen, Eila Pakkala |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Analyte Carbon Isotopes Chromatography Chemistry Coefficient of variation Extraction (chemistry) Catechols Catechol O-Methyltransferase Inhibitors Mass spectrometry Biochemistry Gas Chromatography-Mass Spectrometry Nitecapone chemistry.chemical_compound Pentanones Molecular Medicine Humans Selected ion monitoring Gas chromatography Gas chromatography–mass spectrometry Spectroscopy |
| Zdroj: | Biological mass spectrometry. 20(12) |
| ISSN: | 1052-9306 |
| Popis: | A gas chromatographic/mass spectrometric method is developed and validated for simultaneous determination of nitecapone and its 13C6-labelled analogue in human plasma using (2H6,13C6)nitecapone as internal standard. The method involves extraction of the analytes from plasma to ethyl acetate-hexane mixture (20:80) and conversion to bis(trimethylsilyl) ethers prior to determination by gas chromatography/mass spectrometry using selected ion monitoring. The quantification range is 0.5-2000 ng ml-1. Precision ranges from 11.3% (coefficient of variation) at low levels to 2.4% at high levels. Recovery is about 50% in the whole range. The method is applied to a pharmacokinetic study where nitecapone diluted with 14C-labelled nitecapone is given intravenously concomitantly with an oral dose of (13C6)nitecapone. |
| Databáze: | OpenAIRE |
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