Role of concanavalin A lectin in recognition of pterygium remnant after surgical excision: Preliminary results of a prospective study
| Autor: | Paulina del C. Leyva Bohorquez, Eduardo Pérez-Campos, Pedro Hernández-Cruz, Juan A Díaz-González, Miguel A Mayoral-Chávez, Ruth Martínez-Cruz, Ma del Pilar Gabriel de la Torre |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Photomicrography medicine.medical_specialty Adolescent Pterygium Diagnosis Differential Conjunctival Diseases Concanavalin A lectin Recurrence Concanavalin A medicine Animals Humans Prospective Studies Prospective cohort study Postoperative Care biology business.industry Mitomycin C Reproducibility of Results Middle Aged medicine.disease eye diseases Rats Surgery Disease Models Animal Ophthalmology medicine.anatomical_structure Stretch marks biology.protein Immunohistochemistry Original Article Eyelid Mitogens medicine.symptom business Conjunctiva Follow-Up Studies |
| Zdroj: | Indian Journal of Ophthalmology |
| ISSN: | 0301-4738 |
| Popis: | Background: Pterygium is one of the most common conjunctival diseases among ophthalmic pathologies. The frequency of recurrences is high, either after surgical treatment or after treatment combined with mitomycin C or beta-radiation therapy. Aims: The purpose of this study was to determine whether concanavalin A (ConA) lectin bound to the pterygial surface can be used to detect recurrence or remnants of pterygium after surgical excision. Materials and Methods: This was a prospective study on 20 patients with pterygium, divided in five stages, pre-surgery, early post-surgery (24h), late post-surgery (seven days), very late post-surgery (four weeks) and two months after the procedure. A drop of fluorescein-marked Con A (35 µg/mL) was instilled in the lower conjunctival eyelid sac and the eye was exposed to the light of a Wood's lamp for an average of five seconds. Results: Out of the 20 patients, eight patients were found to have fluorescent stretch marks over the scar corresponding to residual pterygial tissue at four weeks; two months after the procedure of re-surgery we observed no fluorescent remnants. All residual pterygia were confirmed through histochemistry studies. Conclusion: It was possible to detect remnants of pterygium in postoperative patients and recurrences in early pre-clinical stages through the visualization of fluorescent ConA bound to the pterygial surface. |
| Databáze: | OpenAIRE |
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