Thrombopoietin induces histidine decarboxylase gene expression in c-mpl transfected UT7 cells
| Autor: | Michel Dy, Elke Schneider, François Machavoine, Dominique Dumenil, Malvyne Rolli-Derkinderen, Michel Arock, Anne Arnould, Michael Bodger, Najet Debili, Maria Pacilio |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_treatment
Biophysics Gene Expression Biology Basophil Histidine Decarboxylase Transfection Biochemistry chemistry.chemical_compound Megakaryocyte Leukemia Megakaryoblastic Acute Proto-Oncogene Proteins medicine Tumor Cells Cultured Humans RNA Messenger Enzyme Inhibitors Receptors Cytokine Molecular Biology Thrombopoietin Protein Kinase C Mitogen-Activated Protein Kinase Kinases Growth factor hemic and immune systems Cell Differentiation Cell Biology Histidine decarboxylase Cell biology Basophils Neoplasm Proteins medicine.anatomical_structure chemistry embryonic structures Tetradecanoylphorbol Acetate Signal transduction Basophil differentiation Megakaryocytes Receptors Thrombopoietin Histamine Signal Transduction |
| Zdroj: | Biochemical and biophysical research communications. 285(5) |
| ISSN: | 0006-291X |
| Popis: | The leukemic cell line UT7 is endowed with both megakaryocyte and basophil differentiation potential, as judged by its capacity to respond to PMA by displaying megakaryocytic and basophilic markers and to produce histamine by neosynthesis. Herein, we addressed the question whether the biological activities characteristic of basophil differentiation were still induced when c-mpl-transfected UT7 cells received a specific megakaryocytic differentiation signal delivered by thrombopoietin (TPO). Surprisingly, we found that histamine synthesis did effectively occur in response to the growth factor. This activity was not associated with megakaryopoiesis since it was not detected in megakaryocytes generated from CD34+ cells cultured in the presence of TPO. Comparing different c-mpl-transfected cell lines, we found that the amount of histamine generated in response to TPO correlated with their responsiveness to PMA, but not with their level of c-mpl expression, thus revealing an intrinsic basophil differentiation potential. Both PMA- and TPO-induced histamine synthesis was reduced by PKC and MEKs inhibitors, indicating that the induction occurred through a common signalling pathway. |
| Databáze: | OpenAIRE |
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