Osmotic permeability of Novikoff hepatoma cells
| Autor: | Rita Anne Garrick, Thomas G. Polefka, William R. Redwood |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Cell Membrane Permeability
Aqueous solution Membrane permeability Chemistry Diffusion Cell Membrane Osmolar Concentration Biophysics Analytical chemistry Water Cell Biology Activation energy Biochemistry Rats Kinetics Liver Neoplasms Experimental Membrane Hydraulic conductivity Permeability (electromagnetism) Animals Female Lipid bilayer |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 642:79-87 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(81)90139-5 |
| Popis: | The osmotic permeability coefficient (Pf) for water movement across Novikoff hepatoma cells was found to be 82 ± 3 (S.E.) · 10−5 cm · s−1 at 20°C. The corresponding diffusional permeability coefficient for 3HHO (Pd) was 97 ± 10 (S.E.) · 10−5 cm · s−1, therefore the ratio P f P d is close to unity. The apparent activation energy for water filtration was 10.4 ± 0.4 (S.E.) kcal · mol−1. This value is significantly greater than the activation energy for the self diffusion of water. The product of the hydraulic permeability coefficient and the viscosity coefficient for water was temperature-dependent. However, the product of the hydraulic permeability coefficient and the viscosity coefficient for membrane lipid did not vary with temperature. These data are interpreted as evidence for water movement across a lipid membrane barrier rather than through aqueous channels. |
| Databáze: | OpenAIRE |
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