ETHANOL PRECIPITATION AS A DOWNSTREAM PROCESSING STEP FOR CONCENTRATION OF XYLANASES PRODUCED BY SUBMERGED AND SOLID-STATE FERMENTATION
| Autor: | Everson Alves Miranda, Max Adilson Lima Costa, Cristiane S. Farinas |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Central composite design 020209 energy General Chemical Engineering 02 engineering and technology 01 natural sciences 010608 biotechnology 0202 electrical engineering electronic engineering information engineering lcsh:Chemical engineering Ethanol precipitation xylanase Downstream processing biology Chemistry Precipitation (chemistry) Aspergillus niger lcsh:TP155-156 biology.organism_classification Pulp and paper industry downstream processing Solid-state fermentation ethanol precipitation Xylanase Fermentation |
| Zdroj: | Brazilian Journal of Chemical Engineering, Vol 35, Iss 2, Pp 477-488 Brazilian Journal of Chemical Engineering v.35 n.2 2018 Brazilian Journal of Chemical Engineering Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
| ISSN: | 1678-4383 0104-6632 |
| Popis: | Xylanases have applications in different industries, being produced by microbial cultivations using submerged (SmF) or solid-state fermentation (SSF). Precipitation stands out as a potential method for the concentration of xylanases, especially with the use as ethanol as the precipitant due to its compatibility with the biorefinery concept. This paper presents a comparative laboratory scale study of ethanol precipitation of xylanases produced by Aspergillus niger cultivated under SSF and SmF. Precipitation conditions were selected according to a central composite design. Statistical analysis showed a significant effect of pH on the recoveries of total protein and xylanase activity. The kinetic profiles showed that a relatively short period of time (up to 15 min) was sufficient to recover most of the xylanase activity precipitated under the selected conditions. Xylanase recoveries of 65 and 79% were achieved for the SSF and SmF enzymatic complexes, respectively. |
| Databáze: | OpenAIRE |
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