Developmental regulation of neuroligand-induced responses in cultured oligodendroglia
| Autor: | Brigitte Malgrange, Cécile Mazy-Servais, Bernard Rogister, Gustave Moonen, Jean-Michel Rigo, Paul Coucke, Shibeshih Belachew |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Serotonin Patch-Clamp Techniques Cellular differentiation Glycine Glutamic Acid Substance P Biology Membrane Potentials chemistry.chemical_compound Calcium imaging Internal medicine medicine Tumor Cells Cultured Animals Patch clamp Neurotransmitter Cells Cultured gamma-Aminobutyric Acid Cerebral Cortex Neurotransmitter Agents General Neuroscience Glutamate receptor Oligodendrocyte Acetylcholine Rats Oligodendroglia medicine.anatomical_structure Endocrinology chemistry Animals Newborn Neuroglia |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0959-4965 |
| Popis: | USING whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP differentiate into oligodendrocytes (ODC), the amplitude of peak currents elicited by saturating concentrations of these transmitters decreases except for 5-HT. Intracellular Ca 2+ concentration changes induced by microperfusion of glutamate, 5-HT, TRH, met-enkephalin and substance P were monitored using a fluo-3-based calcium imaging system. When OP cells differentiate into ODC, a global decrease of the proportion of responding cells is observed. During type-2 astrocytes commitment, this proportion decreases for 5-HT, TRH- and met-enkephalin stimulations whereas it remains constant for substance P and glutamate. These data demonstrate a development regulation of neurotransmitter- and neuropeptide-induced responses within the oligodendroglial lineage. |
| Databáze: | OpenAIRE |
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