The effect of chlorpyrifos upon ATPase activity of sarcoplasmic reticulum and biomechanics of skeleta l muscle contraction
| Autor: | M S Miroshnychenko, D O Zavodovskiy, V M Soroca, L V Korchins ka, D M Nozdrenko |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Insecticides Contraction (grammar) Muscle Fibers Skeletal Rana temporaria Neuromuscular transmission Biochemistry lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals lcsh:QD415-436 Calcium metabolism Ion Transport Dose-Response Relationship Drug Endoplasmic reticulum Organophosphate Biomechanical Phenomena Dose–response relationship Sarcoplasmic Reticulum 030104 developmental biology chemistry Chlorpyrifos Biophysics Calcium Ca(2+) Mg(2+)-ATPase medicine.symptom 030217 neurology & neurosurgery Muscle contraction Muscle Contraction |
| Zdroj: | Ukrainian Biochemical Journal, Vol 88, Iss 2, Pp 82-88 (2016) |
| ISSN: | 2409-4943 |
| Popis: | We investigated the effect of chlorpyrifos, an organophosphate insecticide, on Ca2+,Mg2+-ATPase activity of sarcoplasmic reticulum and on contraction dynamics (force and length changes) of Rana temporaria m. tibialis anterior muscle fiber bundles. All of the used concentrations of chlorpyrifos (10-6 to 10-5 M) caused decrease of Ca2+,Mg2+-ATPase activity. The inhibition of Ca2+,Mg2+-ATPase activity by chlorpyriphos in concentrations of 10-6 M to 7.5·10-6 M is due to permeation of sarcoplasmic reticulum rather than due to direct enzyme inhibition by organophosphate insecticides. The inhibitory properties of the compound were higher at increased concentration and exposure timeframes. Chlorpyrifos in concentration range of 10-6 to 7.5·10-6 M causes changes in muscle fiber response force that were more pronounced than changes in contractile length. We demonstrated inhibition of Ca2+,Mg2+-ATPase activity caused by noncholinergic effects of chlorpyriphos. It is possible to conclude that influence of organophosphate insecticides happens not only in the neuromuscular transmission but also on the level of subcellular structures. |
| Databáze: | OpenAIRE |
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