Rapid identification of Staphylococcus aureus by using fluorescent staphylocoagulase assays
| Autor: | John D. Perry, F.K. Gould, M.G. Holliday, Marilyn Ford |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
Coagulase Staphylococcus aureus Micrococcaceae Staphylococcal infections medicine.disease_cause Microbiology Coumarins Predictive Value of Tests parasitic diseases medicine Humans Fluorescent Dyes chemistry.chemical_classification Bacteriological Techniques biology business.industry Bacteriology Staphylococcal Infections biology.organism_classification medicine.disease bacterial infections and mycoses Staphylocoagulase Latex fixation test Enzyme chemistry Evaluation Studies as Topic business Oligopeptides Bacteria Latex Fixation Tests |
| Zdroj: | Journal of clinical microbiology. 37(4) |
| ISSN: | 0095-1137 |
| Popis: | Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t -boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t -boc-β-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and latex agglutination (LA) (Sanofi Diagnostics Pasteur, Guildford, Surrey, United Kingdom) by using 406 clinical isolates of staphylococci, and they produced positive and negative predictive values of 99.2 and 99.1% for LA, 98.9 and 92.7% for VPA, and 98.9 and 99.1% for BB. Fluorescent assays used colonies from solid media, thereby eliminating the need for broth cultures, and were performed in microtiter trays, thus making them suitable for large-scale screening. |
| Databáze: | OpenAIRE |
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