Control of Cell Shape, Neurite Outgrowth, and Migration by a Nogo-A/HSPG Interaction

Autor: Anissa Kempf, Rafael D. Fritz, Zorica Ristic, Martin E. Schwab, Antonio Schmandke, Andrea M Kaelin, Valentina Grande, Enrica Boda, Jessica C. F. Kwok, James W. Fawcett, Annalisa Buffo, Olivier Pertz, Bjoern Tews, Andre Schmandke
Přispěvatelé: University of Zurich, Kempf, Anissa, Fawcett, James [0000-0002-7990-4568], Apollo - University of Cambridge Repository
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Nogo Proteins
RHOA
migration
Syndecan 1
1309 Developmental Biology
1307 Cell Biology
chemistry.chemical_compound
Mice
0302 clinical medicine
Cell Movement
Receptor
Cells
Cultured
S1PR2
biology
Heparan sulfate
adhesion
HSPG
neuroblast
nogo-A
outgrowth
RMS
spreading
SVZ
syndecan
Cell biology
Receptors
Lysosphingolipid
Proteoglycans
psychological phenomena and processes
Protein Binding
Neurite
Neuronal Outgrowth
610 Medicine & health
General Biochemistry
Genetics and Molecular Biology
Cell Line
03 medical and health sciences
1300 General Biochemistry
Genetics and Molecular Biology
mental disorders
1312 Molecular Biology
Neurites
Animals
Chondroitin sulfate
Molecular Biology
Cell Shape
10242 Brain Research Institute
Cell Biology
500 Science
carbohydrates (lipids)
030104 developmental biology
chemistry
biology.protein
570 Life sciences
Heparitin Sulfate
Carrier Proteins
030217 neurology & neurosurgery
Heparan Sulfate Proteoglycans
Developmental Biology
DOI: 10.7892/boris.109215
Popis: Heparan sulfate proteoglycans (HSPGs) critically modulate adhesion-, growth-, and migration-related processes. Here, we show that the transmembrane protein, Nogo-A, inhibits neurite outgrowth and cell spreading in neurons and Nogo-A-responsive cell lines via HSPGs. The extracellular, active 180 amino acid Nogo-A region, named Nogo-A-Δ20, binds to heparin and brain-derived heparan sulfate glycosaminoglycans (GAGs) but not to the closely related chondroitin sulfate GAGs. HSPGs are required for Nogo-A-Δ20-induced inhibition of adhesion, cell spreading, and neurite outgrowth, as well as for RhoA activation. Surprisingly, we show that Nogo-A-Δ20 can act via HSPGs independently of its receptor, Sphingosine-1-Phosphate receptor 2 (S1PR2). We thereby identify the HSPG family members syndecan-3 and syndecan-4 as functional receptors for Nogo-A-Δ20. Finally, we show in explant cultures ex vivo that Nogo-A-Δ20 promotes the migration of neuroblasts via HSPGs but not S1PR2.
Databáze: OpenAIRE
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