Voltammetric Sensor for Total Cholesterol Determination
| Autor: | Elena V. Dorozhko, K. V. Derina, Darya A. Vishenkova, Olesya Voronova, Elena Korotkova |
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| Jazyk: | angličtina |
| Předmět: |
Analyte
Chemistry(all) Cholesterol oxidase Analytical chemistry hydrogen peroxide Electrochemistry biosensor Horseradish peroxidase вольтамперометрия cholesterol oxidase Graphite холестерин Chromatography biology horseradish peroxidase Chemistry technology industry and agriculture cholesterol General Medicine вольтамперометрические датчики Hydrogen peroxide биосенсоры Cholesterol Linear range Linear sweep voltammetry Chemical Engineering(all) biology.protein перекись водорода Biosensor |
| Zdroj: | Procedia Chemistry. :513-518 |
| ISSN: | 1876-6196 |
| DOI: | 10.1016/j.proche.2014.10.087 |
| Popis: | We report on a voltammetric sensor for the detection of total cholesterol. The sensor was fabricated by co-immobilization of two enzymes: cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) on porous graphite. The electrochemical behavior of the sensor was studied with the use of linear sweep voltammetry. It has been shown that the sensor has high stability and high sensitivity (16 μA mM −1 cm −2 ). The biosensor exhibited a wide linear range up to 300 mol/dm 3 in a condition close to physiological (pH=6.86). Besides, the interferences of some key analytes containing in the blood were studied. As a matter of fact, making a fabricated sensor is rather promising for using in clinical practice. |
| Databáze: | OpenAIRE |
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