Swimming attenuates inflammation, oxidative stress, and apoptosis in a rat model of dextran sulfate sodium-induced chronic colitis
| Autor: | Jun-Tang Li, Lifeng Wang, Ya-Li Zhao, Ning-Ning Liu, Zhiqiang Yao, Xiao-Shan Zhu, Ling Qin, Angang Yang, Chun-Fang Gao, Qi Chang, Qin-Qin Liu |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Time Factors CD3 Complex T-Lymphocytes medicine.medical_treatment Apoptosis Pharmacology Weight Gain medicine.disease_cause Antioxidants Rats Sprague-Dawley chemistry.chemical_compound oxidative stress swimming chemistry.chemical_classification biology Glutathione peroxidase Dextran Sulfate Research Paper: Immunology Colitis Malondialdehyde Exercise Therapy Cytokine Neutrophil Infiltration Oncology Immunology and Microbiology Section Inflammation Mediators medicine.symptom Signal Transduction Colon Inflammation Superoxide dismutase 03 medical and health sciences medicine Animals Immune response business.industry Immunity chronic colitis medicine.disease Disease Models Animal 030104 developmental biology chemistry inflammation Chronic Disease Splenomegaly Immunology biology.protein Calprotectin Apoptosis Regulatory Proteins Reactive Oxygen Species business Biomarkers Oxidative stress |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Ling Qin 1,* , Zhi-qiang Yao 2,* , Qi Chang 3,* , Ya-li Zhao 2,* , Ning-ning Liu 2,* , Xiao-shan Zhu 2 , Qin-qin Liu 2 , Li-feng Wang 4 , An-gang Yang 5 , Chun-fang Gao 2 and Jun-tang Li 2,3,4,5 1 Department of Hematology, First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan, China 2 Centre of Inflammation and Cancer Research, 150th Central Hospital of PLA, Luoyang, Henan, China 3 Centre of Biomaterial and Biophysics Research, Institute of Training Medicine, 150th Central Hospital of PLA, Luoyang, Henan, China 4 State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an, Shaanxi, China 5 State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi’an, Shaanxi, China * These authors have contributed equally to this work Correspondence to: Jun-tang Li, email: // Chun-fang Gao, email: // Ling Qin, email: // Keywords : chronic colitis, swimming, inflammation, oxidative stress, apoptosis, Immunology and Microbiology Section, Immune response, Immunity Received : March 28, 2016 Accepted : December 15, 2016 Published : December 21, 2016 Abstract Increasing evidence suggests that regular physical exercise suppresses chronic inflammation. However, the potential inhibitory effects of swimming on dextran sulfate sodium (DSS)-induced chronic colitis, and its underlying mechanisms, remain unclear. In this study, rats were orally administered DSS to induce chronic colitis, and subsequently treated with or without swimming exercise. A 7-week swimming program (1 or 1.5 hours per day, 5 days per week) ameliorated DSS-caused colon shortening, colon barrier disruption, spleen enlargement, serum LDH release, and reduction of body weight gain. Swimming for 1.5 hours per day afforded greater protection than 1 hour per day. Swimming ameliorated DSS-induced decrease in crypt depth, and increases in myeloperoxidase activity, infiltration of Ly6G + neutrophils and TNF-α- and IFN-γ-expressing CD3 + T cells, as well as fecal calprotectin and lactoferrin. Swimming inhibited pro-inflammatory cytokine and chemokine production and decreased the protein expression of phosphorylated nuclear factor-κB p65 and cyclooxygenase 2, whereas it elevated interleukin-10 levels. Swimming impeded the generation of reactive oxygen species, malondialdehyde, and nitric oxide; however, it boosted glutathione levels, total antioxidant capacity, and superoxide dismutase and glutathione peroxidase activities. Additionally, swimming decreased caspase-3 activity and expression of apoptosis-inducing factor, cytochrome c, Bax, and cleaved-caspase-3, but increased Bcl-2 levels. Overall, these results suggest that swimming exerts beneficial effects on DSS-induced chronic colitis by modulating inflammation, oxidative stress, and apoptosis. |
| Databáze: | OpenAIRE |
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