Choline metabolome response to prenatal choline supplementation across pregnancy: A randomized controlled trial
| Autor: | Siraphat Taesuwan, Ramesh Vidavalur, Olga V. Malysheva, Kevin C. Klatt, Srisatish Devapatla, Marie A. Caudill, Julie E.H. Nevins, Melissa Q. McDougall, Erica Bender |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
medicine.medical_specialty Placenta Biochemistry Choline Dimethylglycine Young Adult chemistry.chemical_compound Fetus Pregnancy Phosphatidylcholine Internal medicine Genetics medicine Metabolome Humans Molecular Biology business.industry Fetal Blood medicine.disease Adaptation Physiological Endocrinology chemistry Case-Control Studies Dietary Supplements Gestation Female business Biotechnology Choline chloride |
| Zdroj: | The FASEB Journal. 35 |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | Pregnancy places a unique stress upon choline metabolism, requiring adaptations to support both maternal and fetal requirements. The impact of pregnancy and prenatal choline supplementation on choline and its metabolome in free-living, healthy adults is relatively uncharacterized. This study investigated the effect of prenatal choline supplementation on maternal and fetal biomarkers of choline metabolism among free-living pregnant persons consuming self-selected diets. Participants were randomized to supplemental choline (as choline chloride) intakes of 550 mg/d (500 mg/d d0-choline + 50 mg/d methyl-d9-choline; intervention) or 25 mg/d d9-choline (control) from gestational week (GW) 12-16 until Delivery. Fasting blood and 24-h urine samples were obtained at study Visit 1 (GW 12-16), Visit 2 (GW 20-24), and Visit 3 (GW 28-32). At Delivery, maternal and cord blood and placental tissue samples were collected. Participants randomized to 550 (vs. 25) mg supplemental choline/d achieved higher (p < .05) plasma concentrations of free choline, betaine, dimethylglycine, phosphatidylcholine (PC), and sphingomyelin at one or more study timepoint. Betaine was most responsive to prenatal choline supplementation with increases (p ≤ .001) in maternal plasma observed at Visit 2-Delivery (relative to Visit 1 and control), as well as in the placenta and cord plasma. Notably, greater plasma enrichments of d3-PC and LDL-C were observed in the intervention (vs. control) group, indicating enhanced PC synthesis through the de novo phosphatidylethanolamine N-methyltransferase pathway and lipid export. Overall, these data show that prenatal choline supplementation profoundly alters the choline metabolome, supporting pregnancy-related metabolic adaptations and revealing biomarkers for use in nutritional assessment and monitoring during pregnancy. |
| Databáze: | OpenAIRE |
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