Acanthoscurria gomesiana spider-derived synthetic mygalin in the dorsal raphe nucleus modulates acute and chronic pain
| Autor: | Ana C. Medeiros, Renato Leonardo de Freitas, Pedro Ismael da Silva Júnior, Norberto Cysne Coimbra, Wagner Ferreira dos Santos, Priscila Medeiros |
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| Rok vydání: | 2021 |
| Předmět: |
Dorsal Raphe Nucleus
Male Microinjections Spermidine Synthetic Drugs Health Toxicology and Mutagenesis Analgesic Pharmacology Toxicology Biochemistry chemistry.chemical_compound Dorsal raphe nucleus Hemolymph Medicine Animals Rats Wistar Molecular Biology Analgesics business.industry Chronic pain Spiders General Medicine medicine.disease Acute Pain Rats Disease Models Animal RESULTADO DE TRATAMENTO Nociception Treatment Outcome chemistry Neuropathic pain CNQX Molecular Medicine Neuralgia NBQX Sciatic nerve Chronic Pain business |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1099-0461 |
| Popis: | Mygalin, a diacylspermidine that is naturally found in the hemolymph of the spider Acanthoscurria gomesiana, is of interest for development as a potential analgesic. Previous studies have shown that acylpolyamines modulate glutamatergic receptors with the potential to alter pain pathways. This study aimed to evaluate the effects of mygalin on acute and chronic pain in rodents. For evaluation of acute pain, Wistar rats were subjected to tail-flick and hot-plate nociceptive tests. For the evaluation of chronic neuropathic pain, a partial ligation of the sciatic nerve was performed and, 21 days later, animals were examined in hot-plate, tail-flick, acetone, and von Frey tests. Either Mygalin or vehicle was microinjected in the dorsal raphe nucleus (DRN) before the tests. Another group was pretreated with selective antagonists of glutamate receptors (LY 235959, MK-801, CNQX, and NBQX). Mygalin decreases nociceptive thresholds on both acute and chronic neuropathic pain models in all the tests performed. The lowest dose of mygalin yielded the most effective nociception, showing an increase of 63% of the nociceptive threshold of animals with neuropathic chronic pain. In conclusion, mygalin microinjection in the DRN results in antinociceptive effect in models of neuropathic pain, suggesting that acylpolyamines and their derivatives, such as this diacylspermidine, could be pursued for the treatment of neuropathic pain and development of selective analgesics. |
| Databáze: | OpenAIRE |
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