Porous tantalum stimulates the proliferation and osteogenesis of osteoblasts from elderly female patients
| Autor: | Ankur Jhaveri, Karen Sagomonyants, Michael S. Aronow, Gloria Gronowicz, Mariam Hakim-Zargar |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
medicine.medical_specialty Biocompatible Materials Tantalum Young Adult Tissue culture Osteogenesis Internal medicine Female patient medicine Humans Orthopedics and Sports Medicine Cell adhesion Cells Cultured Aged Cell Proliferation Titanium Osteoblasts Chemistry Cell growth Porous tantalum Calcinosis Osteoblast Middle Aged In vitro Surgery medicine.anatomical_structure Endocrinology Female Implant Biomarkers |
| Zdroj: | Journal of Orthopaedic Research. 29:609-616 |
| ISSN: | 0736-0266 |
| Popis: | Porous tantalum (Ta) implants have been successful in various orthopedic procedures for patients with compromised bone-forming abilities. Previous studies demonstrated that human osteoblast (HOB) cultures from older female patients produced less bone on implant materials in vitro compared to HOBs from age-matched male and younger female patients. In this study, the responses of HOBs from younger (45) and older (60 years old) female patients were compared on Ta, titanium fiber mesh (TFM) and tissue culture plastic. Adhesion, proliferation, and mineralization were greater in cells from younger patients than from older patients. Cell adhesion was slightly higher on Ta than TFM or plastic. However, Ta highly stimulated cell proliferation with a 4- and 6-fold increase compared to TFM for cells from younger and older patients, respectively, and 12- and 16-fold increase in proliferation compared to cells on plastic (p ≤ 0.001). At 3 weeks, mineralization was significantly higher on Ta compared to TFM for HOBs from older patients (p ≤ 0.05). Expression levels of bone matrix markers demonstrated differences dependent on age and substrate. Scanning electron micrographs revealed HOBs covering the surfaces and entering the pores of both Ta and TFM. In conclusion, tantalum greatly stimulates cell proliferation, and improves the ability of HOBs from older patients to form bone. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text