Experimental and calculated parameters on particle phagocytosis by alveolar macrophages
| Autor: | Margot Lundborg, N. T. Gross, Per Camner, Connie Jarstrand, Per Gerde, Lena Låstbom |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Time Factors Physiology Silicon dioxide Phagocytosis Receptors Cell Surface Inflammation Receptors Fc Microbiology Rats Sprague-Dawley Interferon-gamma chemistry.chemical_compound Physiology (medical) Candida albicans Macrophages Alveolar Cell Adhesion medicine Animals Macrophage Lectins C-Type Receptors Immunologic Cells Cultured Receptors Lipoprotein Receptors Scavenger biology digestive oral and skin physiology Membrane Proteins Scavenger Receptors Class B Silicon Dioxide biology.organism_classification Recombinant Proteins Corpus albicans Rats Mannose-Binding Lectins medicine.anatomical_structure chemistry Cryptococcus neoformans Biophysics Particle Pulmonary alveolus medicine.symptom Mannose Receptor |
| Zdroj: | Journal of Applied Physiology. 92:2608-2616 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/japplphysiol.01067.2001 |
| Popis: | Phagocytosis of three types of fluorescein-labeled test particles by rat alveolar macrophages (AM) were studied: spherical silica (3.2 microm), heat-killed Candida albicans (3.8 microm), and heat-killed Cryptococcus neoformans (6.1 microm) opsonized with specific IgG. These particles should attach to scavenger, mannose, and Fc receptors, respectively. Both control AM and AM pretreated for 20 h with interferon-gamma (12.5 or 50 U/ml) were studied. The sum of the number of attached and ingested particles per AM (accumulated attachment) was used as a measure of the attachment process, and the number of ingested particles per AM divided by the accumulated attachment (ingested fraction) was used as a measure of the ingestion process. The average ingestion time (IT), which is also a measure of the ingestion process, was calculated from the experimental data. The ingestion process was independent of the attachment process. IT increased with the time of observation. This is explained by the fact that IT determined from observation times shorter than the whole distribution of IT for a certain particle results in a shorter IT than the real average IT. C. albicans (mannose receptor) had the fastest ingestion process, C. neoformans opsonized with specific IgG (Fc receptor) had ingestion that was nearly as fast, and the silica particles (scavenger receptors) had the slowest ingestion process. Treatment with interferon-gamma markedly impaired the attachment process for all three types of particles (and three types of receptors) but clearly impaired the ingestion process only for silica particles (scavenger receptors). |
| Databáze: | OpenAIRE |
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