In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach
| Autor: | Mathieu Vinken, Andrés Tabernilla, Kaat Leroy, Raf Van Campenhout, Emma Arnesdotter, Bruna dos Santos Rodrigues, Eva Gijbels, Alanah Pieters, Anne Caufriez, Axelle Cooreman, Ana Rita Coelho Gomes |
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| Přispěvatelé: | Pharmaceutical and Pharmacological Sciences, Faculty of Medicine and Pharmacy, Experimental in vitro toxicology and dermato-cosmetology, Liver Connexin and Pannexin Research Group, Connexin Signalling Research Group |
| Rok vydání: | 2021 |
| Předmět: |
Liver toxicity
QH301-705.5 education Review liver-specific toxicity In Vitro Techniques Bioinformatics liver Risk Assessment Catalysis Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Toxicity Tests Medicine Animals Humans Human safety Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy health care economics and organizations 030304 developmental biology 0303 health sciences mechanisms business.industry Organic Chemistry In vitro toxicology in vitro General Medicine In vitro 3. Good health Computer Science Applications Chemistry 030220 oncology & carcinogenesis Toxicity Models Animal cytotoxicity Chemical and Drug Induced Liver Injury business |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 5038, p 5038 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22095038 |
| Popis: | The liver is among the most frequently targeted organs by noxious chemicals of diverse nature. Liver toxicity testing using laboratory animals not only raises serious ethical questions, but is also rather poorly predictive of human safety towards chemicals. Increasing attention is, therefore, being paid to the development of non-animal and human-based testing schemes, which rely to a great extent on in vitro methodology. The present paper proposes a rationalized tiered in vitro testing strategy to detect liver toxicity triggered by chemicals, in which the first tier is focused on assessing general cytotoxicity, while the second tier is aimed at identifying liver-specific toxicity as such. A state-of-the-art overview is provided of the most commonly used in vitro assays that can be used in both tiers. Advantages and disadvantages of each assay as well as overall practical considerations are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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