Browplasminin, a condensed tannin with anti-plasmin activity isolated from an aqueous extract of Brownea grandiceps Jacq. flowers
| Autor: | Eva Vonasek, Betzabeth Pereira, Mónica Rincón, Josmary Brazón |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Plasmin medicine.medical_treatment Decoction Flowers 01 natural sciences Fibrin 03 medical and health sciences chemistry.chemical_compound Inhibitory Concentration 50 Antifibrinolytic agent Drug Discovery Fibrinolysis medicine Fibrinolysin Chromatography High Pressure Liquid Pharmacology Chromatography biology Dose-Response Relationship Drug 010405 organic chemistry Plant Extracts Catechin Fabaceae Trypsin biology.organism_classification Venezuela Antifibrinolytic Agents Hydrolyzable Tannins 0104 chemical sciences 030104 developmental biology Biochemistry chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Medicine Traditional Brownea medicine.drug |
| Zdroj: | Journal of ethnopharmacology. 198 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Following Venezuelan traditional medicine, females with heavy menstrual blood loss (menorrhagia) drink Brownea grandiceps Jacq. flowers (BG) decoctions to reduce the bleeding. In a previous study, we demonstrated that BG aqueous extract (E) possesses a potent anti-fibrinolytic activity capable of inhibiting plasmin, the main serine-protease that degrades fibrin. It is widely known that plasmin inhibitors are often used as anti-fibrinolytics to reduce bleeding during surgeries with high risk of blood loss such as cardiac, liver, vascular, tooth extraction and large orthopedic procedures, as well as for menorrhagia treatments. The aim of this work was to isolate and characterize from BGE the compound responsible for the reported anti-fibrinolytic activity. Materials and methods A decoction of BG was prepared; then it was homogenized, centrifuged and lyophilized to obtain BGE. Subsequently the extract was fractionated by gel filtration and reverse phase using HPLC and the active compound was characterized by MALDI-ToF MS. The kinetic parameters of anti-plasmin activity were evaluated by an amidolytic assay using a chromogenic substrate; also the anti-plasmin activity was estimated by fibrin plate method. Data were analyzed by nonparametric statistics. Results The active compound was a condensed tannin denominated Browplasminin, which is capable of inhibiting the plasmin activity in a dose-dependent manner when measured in fibrin plates or by the amidolytic activity method; it also has a minor effect on the FXa activity. However, it does not affect the activity of other serine-proteases such as trypsin, t-PA or u-PA. Browplasminin consists predominately of heteroflavan-3-ols of catechin with B-type linkages, and extents up to heptadecamers (~ 5000 Da), with hexose residues attached to the polymer that presents a high degree of galloylation. Its IC50 for plasmin was 47.80 μg/mL and for FXa was 237.08 μg/mL, while the Ki were 0.76 and 61.61 μg/mL for plasmin and FXa, respectively. Conclusions The overall outcome of this study suggests that Browplasminin could be responsible for reducing heavy menstrual bleeding in women because its kinetic parameters showed that is a good plasmin inhibitor. |
| Databáze: | OpenAIRE |
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