Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23

Autor: Ole Lagatie, Zeleke Mekonnen, Daniel Dana, Bruno Levecke, Bieke Van Dorst, Lieven J. Stuyver, Ann Verheyen, Johnny Vlaminck
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Helminthiasis
WUCHERERIA-BANCROFTI
Onchocerciasis
Serology
Epitopes
Feces
Soil
0302 clinical medicine
Seroepidemiologic Studies
Medicine and Health Sciences
ASSAY
Child
biology
Soil-transmitted helminths
Infectious Diseases
Schistosoma mansoni
Adult
Adolescent
030231 tropical medicine
Antibodies
Helminth
Enzyme-Linked Immunosorbent Assay
DIAGNOSIS
ANTIGENS
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Young Adult
Antigen
SURVEILLANCE
parasitic diseases
medicine
Seroprevalence
Helminths
Animals
Humans
lcsh:RC109-216
Seroconversion
Linear epitope
Research
Biology and Life Sciences
PERFORMANCE
biology.organism_classification
medicine.disease
Virology
Onchocerca volvulus
Schistosomiasis mansoni
030104 developmental biology
River blindness
Parasitology
Ethiopia
Zdroj: Parasites & Vectors
PARASITES & VECTORS
Parasites & Vectors, Vol 12, Iss 1, Pp 1-7 (2019)
ISSN: 1756-3305
Popis: Background Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. Recently, two linear epitopes encoded in OvMP-1 and OvMP-23 peptides were introduced as serological markers, but the observed antibody cross-reactivity in samples originating from Onchocerca volvulus non-endemic areas required further investigation. Methods We evaluated both peptide markers in an O. volvulus hypo-endemic setting in Jimma Town, Ethiopia using peptide ELISA. For all individuals (n = 303), the infection status with soil-transmitted helminths and Schistosoma mansoni was known. Results We found that 11 (3.6%) individuals were positive for anti-Ov16 IgG4 antibodies, while 34 (11.2%) and 15 (5.0%) individuals were positive for OvMP-1 and OvMP-23, respectively. Out of the 34 OvMP-1 positive samples, 33 were negative on the Ov16 IgG4 ELISA. Similarly, out of the 15 OvMP-23 positive samples, 14 scored negative on this reference method. No difference in seroprevalence for all three markers could be observed between uninfected individuals and individuals infected with different soil-transmitted helminths or S. mansoni. Moreover, the intensity of the response to OvMP-1, OvMP-23 or Ov16 was not significantly stronger in individuals carrying patent STH or S. mansoni infections, nor was there any correlation between the intensities of the responses to the three different antigens. Conclusions This study demonstrates that a patent infection with either soil-transmitted helminths or S. mansoni does not lead to increased antibody recognition of both OvMP-1 and OvMP23. Electronic supplementary material The online version of this article (10.1186/s13071-019-3308-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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