Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23
Autor: | Ole Lagatie, Zeleke Mekonnen, Daniel Dana, Bruno Levecke, Bieke Van Dorst, Lieven J. Stuyver, Ann Verheyen, Johnny Vlaminck |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male Helminthiasis WUCHERERIA-BANCROFTI Onchocerciasis Serology Epitopes Feces Soil 0302 clinical medicine Seroepidemiologic Studies Medicine and Health Sciences ASSAY Child biology Soil-transmitted helminths Infectious Diseases Schistosoma mansoni Adult Adolescent 030231 tropical medicine Antibodies Helminth Enzyme-Linked Immunosorbent Assay DIAGNOSIS ANTIGENS lcsh:Infectious and parasitic diseases 03 medical and health sciences Young Adult Antigen SURVEILLANCE parasitic diseases medicine Seroprevalence Helminths Animals Humans lcsh:RC109-216 Seroconversion Linear epitope Research Biology and Life Sciences PERFORMANCE biology.organism_classification medicine.disease Virology Onchocerca volvulus Schistosomiasis mansoni 030104 developmental biology River blindness Parasitology Ethiopia |
Zdroj: | Parasites & Vectors PARASITES & VECTORS Parasites & Vectors, Vol 12, Iss 1, Pp 1-7 (2019) |
ISSN: | 1756-3305 |
Popis: | Background Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. Recently, two linear epitopes encoded in OvMP-1 and OvMP-23 peptides were introduced as serological markers, but the observed antibody cross-reactivity in samples originating from Onchocerca volvulus non-endemic areas required further investigation. Methods We evaluated both peptide markers in an O. volvulus hypo-endemic setting in Jimma Town, Ethiopia using peptide ELISA. For all individuals (n = 303), the infection status with soil-transmitted helminths and Schistosoma mansoni was known. Results We found that 11 (3.6%) individuals were positive for anti-Ov16 IgG4 antibodies, while 34 (11.2%) and 15 (5.0%) individuals were positive for OvMP-1 and OvMP-23, respectively. Out of the 34 OvMP-1 positive samples, 33 were negative on the Ov16 IgG4 ELISA. Similarly, out of the 15 OvMP-23 positive samples, 14 scored negative on this reference method. No difference in seroprevalence for all three markers could be observed between uninfected individuals and individuals infected with different soil-transmitted helminths or S. mansoni. Moreover, the intensity of the response to OvMP-1, OvMP-23 or Ov16 was not significantly stronger in individuals carrying patent STH or S. mansoni infections, nor was there any correlation between the intensities of the responses to the three different antigens. Conclusions This study demonstrates that a patent infection with either soil-transmitted helminths or S. mansoni does not lead to increased antibody recognition of both OvMP-1 and OvMP23. Electronic supplementary material The online version of this article (10.1186/s13071-019-3308-z) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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