Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies
| Autor: | A. Domingo, Isabel Ortiz, V. Gómez-Arrones, C. Consuegra, M. Diaz-Jimenez, J.J. Carrasco, Manuel Hidalgo, M. Bottrel, Jesús Dorado, B. Pereira |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
animal structures
Embryo quality Embryo recovery rate Biology Article donkey Andrology Recovery rate Donkey lcsh:Zoology lcsh:QL1-991 embryo transfer Estrous cycle Developmental stage embryo recovery rate lcsh:Veterinary medicine General Veterinary Embryo Embryo transfer embryonic structures Herd embryo quality lcsh:SF600-1100 Animal Science and Zoology |
| Zdroj: | Animals 10(11), 1967 (2020) Animals, Vol 10, Iss 1967, p 1967 (2020) Helvia. Repositorio Institucional de la Universidad de Córdoba instname Animals Volume 10 Issue 11 Helvia: Repositorio Institucional de la Universidad de Córdoba Universidad de Córdoba Animals : an Open Access Journal from MDPI |
| Popis: | Embryo transfer and the vitrification of embryos could be used for the conservation and recovery of endangered donkey breeds. It is important to develop techniques that optimize recovery rates and the cryotolerance of donkey embryos. This study evaluates factors affecting the recovery rate, quality, and diameter of embryos obtained from donor jennies as a starting point for the use of vitrification and embryo transfer in the conservation of the Andalusian donkey. A total of 100 embryos were recovered out of 124 estrous cycles (80.6%). The donor jenny affected the rates of positive flushings (PFR p = 0.040) and embryo recovery (ERR p < 0.05) as well as embryo quality (p = 0.004). ERR was also affected by the number of flushings (p < 0.001), donor age (p < 0.05), successive cycle within donor (p < 0.001), and jacks (p < 0.05). Number of flushings (p < 0.001) and jack (p < 0.05) had a significant effect on PFR, whereas the day of flushing influenced the developmental stage (p < 0.001), embryo quality (p < 0.05), and diameter of embryos (p < 0.001). The number of flushings significantly influenced the diameter (p = 0.038) and embryo developmental stage (p = 0.001), whereas the developmental stage was statistically different between herds (p = 0.020). The factors influencing the success of this assisted reproductive technique were donor jenny, donor age, successive cycle within donor, day of flushing, number of flushings, and jack. The identification of these key points is crucial to achieve a higher efficiency of embryo transfer and vitrification processes, before considering their application in the conservation of endangered donkey breeds. |
| Databáze: | OpenAIRE |
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