Properties of GABAergic Neurons Containing Calcium-Permeable Kainate and AMPA-Receptors
| Autor: | Sultan Tuleukhanov, A. I. Sergeev, L. P. Dolgacheva, Valery P. Zinchenko, Artem M. Kosenkov, S. G. Gaidin |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Agonist
medicine.drug_class Science Kainate receptor AMPA receptor Inhibitory postsynaptic potential General Biochemistry Genetics and Molecular Biology Article Glutamatergic chemistry.chemical_compound GABAergic neurons GluA2 subunit GluK1 subunit Ca2+-permeable kainate receptors Ca2+-permeable AMPA receptors spontaneous synchronous activity medicine Neurotransmitter Receptor Ecology Evolution Behavior and Systematics Chemistry musculoskeletal neural and ocular physiology Paleontology Cell biology nervous system Space and Planetary Science GABAergic |
| Zdroj: | Life, Vol 11, Iss 1309, p 1309 (2021) Life Life; Volume 11; Issue 12; Pages: 1309 |
| ISSN: | 2075-1729 |
| Popis: | Calcium-permeable kainate and AMPA receptors (CP-KARs and CP-AMPARs), as well as NMDARs, play a pivotal role in plasticity and in regulating neurotransmitter release. Here we visualized in the mature hippocampal neuroglial cultures the neurons expressing CP-AMPARs and CP-KARs. These neurons were visualized by a characteristic fast sustained [Ca2+]i increase in response to the agonist of these receptors, domoic acid (DoA), and a selective agonist of GluK1-containing KARs, ATPA. Neurons from both subpopulations are GABAergic. The subpopulation of neurons expressing CP-AMPARs includes a larger percentage of calbindin-positive neurons (39.4 ± 6.0%) than the subpopulation of neurons expressing CP-KARs (14.2 ± 7.5% of CB+ neurons). In addition, we have shown for the first time that NH4Cl-induced depolarization faster induces an [Ca2+]i elevation in GABAergic neurons expressing CP-KARs and CP-AMPARs than in most glutamatergic neurons. CP-AMPARs antagonist, NASPM, increased the amplitude of the DoA-induced Ca2+ response in GABAergic neurons expressing CP-KARs, indicating that neurons expressing CP-AMPARs innervate GABAergic neurons expressing CP-KARs. We assume that CP-KARs in inhibitory neurons are involved in the mechanism of outstripping GABA release upon hyperexcitation. |
| Databáze: | OpenAIRE |
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