The liver protection of propylene glycol alginate sodium sulfate preconditioning against ischemia reperfusion injury: focusing MAPK pathway activity
| Autor: | Sainan Li, Ning Liu, Tong Liu, Weiqi Dai, Xiya Lu, Qiang Yu, Fan Wang, Yujing Xia, Jiao Feng, Wenwen Wang, Jingjing Li, Xiaoming Fan, Chuanyong Guo, Ling Xu, Peiqin Niu, Shizan Xu, Kan Chen, Liwei Wu, Wenhui Mo |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine MAPK/ERK pathway Alginates p38 mitogen-activated protein kinases Ischemia lcsh:Medicine Pharmacology Article Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Ischemic Preconditioning lcsh:Science Protein kinase A Mice Inbred BALB C Multidisciplinary Histocytochemistry Chemistry Kinase Gene Expression Profiling Liver Diseases lcsh:R medicine.disease Disease Models Animal 030104 developmental biology Biochemistry Apoptosis Reperfusion Injury 030220 oncology & carcinogenesis Cytokines lcsh:Q Mitogen-Activated Protein Kinases Reperfusion injury Blood Chemical Analysis Signal Transduction |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Hepatic ischemia reperfusion (IR) injury contributes to the morbidity and mortality associated with liver surgery. This study investigated the protective function and mechanism of propylene glycol alginate sodium sulfate (PSS), a sulfated polysaccharide, in a mouse hepatic IR injury model. PSS (25 or 50 mg/kg) or saline were injected intraperitoneally to male Balb/c mice 1 h before 45 min of 70% warm hepatic ischemia and 2, 8, and 24 h of reperfusion. Serum and liver tissue samples were collected for evaluation of hepatocellular damage, liver histology, and assay of inflammatory cytokines, apoptosis- and autophagy-related proteins, and proteins in the mitogen-activated protein kinase (MAPKs). Histological injury and release of transaminases, and inflammatory cytokine production were significantly reduced by PSS pretreatment. The expression of apoptosis- and autophagy-related proteins, and the activation of MAPK signal, including jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and P38 were all affected by PSS treatment compared with IR model controls. PSS protected the liver from IR injury by suppressing the MAPK signaling and down-regulating inflammation, apoptosis, and autophagy. |
| Databáze: | OpenAIRE |
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