Novel HLA-DPB1 alleles detected in the Ethiopian population
| Autor: | Jemal Abdulkadir, W. Verduyn, L.F. Versluis, Marcel G.J. Tilanus, Marius J. Giphart |
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| Rok vydání: | 1995 |
| Předmět: |
Genetics
Cloning education.field_of_study HLA-DP Antigens HLA-DPB1 Base Sequence Immunology Population Molecular Sequence Data General Medicine Biology Hypervariable region Exon Polymorphism (computer science) Immunology and Allergy Humans Typing Amino Acid Sequence Ethiopia Allele education Alleles HLA-DP beta-Chains |
| Zdroj: | Human immunology. 42(2) |
| ISSN: | 0198-8859 |
| Popis: | The number of identified HLA-DPB1 alleles increased rapidly by application of DNA-based typing techniques. PCR-SSO typing indicated the presence of possible new HLA-DPB1 variants in the Ethiopian population. The use of the SBT technique, which considers polymorphic as well as constant regions in the second exon of HLA genes, allowed direct identification of two new allelic variants. Moreover, a recently identified HLA-DPA1 variant was also present in this population. The newly defined allelic HLA-DPB 1 sequences found in five individuals of the Ethiopian population were confirmed by cloning and subsequent sequencing of the cloned DNA. One of the new alleles was shown to segregate in a family and was also present in unrelated individuals. Both new DPB1 alleles represent new combinations of existing polymorphism in the hypervariable regions. In different populations the frequency of these new HLA-DP variants remains to be determined. |
| Databáze: | OpenAIRE |
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