Intestinal Cytokine Response of Commercial Source Broiler Chicks to Salmonella Typhimurium Infection
| Autor: | Edwin T. Moran, Peter S. Holt, Y. O. Fasina, R. W. Moore, D. E. Conner, S. R. McKee |
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| Rok vydání: | 2008 |
| Předmět: |
Salmonella typhimurium
Salmonella medicine.medical_specialty animal structures medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Microbiology Proinflammatory cytokine Downregulation and upregulation Internal medicine medicine Animals Poultry Diseases Salmonella Infections Animal Messenger RNA Broiler Interleukin General Medicine Antibodies Bacterial Immunoglobulin A Endocrinology Cytokine Immunoglobulin G biology.protein Cytokines Animal Science and Zoology Antibody Chickens |
| Zdroj: | Poultry Science. 87:1335-1346 |
| ISSN: | 0032-5791 |
| DOI: | 10.3382/ps.2007-00526 |
| Popis: | Development of molecular-based immunotherapeutic strategies for controlling Salmonella Typhimurium (ST) infection in poultry requires a better understanding of intestinal and cecal cytokine responses. Accordingly, an experiment was conducted to measure changes in intestinal cytokine expression when commercial source broiler chickens were challenged with a nalidixic acid-resistant ST. Ross broiler chicks were nonchallenged with ST (control treatment) or challenged by orally giving 7.8 x 10(6) cfu at 4 d of age (STC treatment). Each treatment consisted of 4 replicate pens with 14 chicks per pen. Expression levels of proinflammatory cytokines, interferon-gamma, and antiinflammatory interleukin (IL)-10 were determined at 5 and 10 d postchallenge (PC). Intestinal flushes were also collected from each treatment at 7 d PC to estimate IgA and IgG. Results showed an upregulation in IL-1beta mRNA in STC chicks at 5 d PC. By 10 d PC, the expression of IL-1beta was further increased and accompanied by an upregulation of IL-6 and interferon-gamma mRNA, whereas IL-10 mRNA expression decreased. It was concluded that ST induced an intestinal mucosal inflammatory response in commercial source broiler chicks less than 2 wk of age. |
| Databáze: | OpenAIRE |
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