Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
Autor: | Sina Dadgar, Barbara Mallmann, Ahmed E. Dhamad, Narasimhan Rajaram, Sami Dridi, Elizabeth S. Greene |
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Rok vydání: | 2021 |
Předmět: |
medicine.medical_specialty
Hot Temperature Meat Diffuse reflectance infrared fourier transform Science Optical spectroscopy chemistry.chemical_element 01 natural sciences Oxygen Article Poultry 010309 optics Hemoglobins Internal medicine Animal physiology 0103 physical sciences medicine Animals Breast Oxygen saturation (medicine) Oxygenated Hemoglobin Multidisciplinary Spectrum Analysis 0402 animal and dairy science 04 agricultural and veterinary sciences Animal Feed 040201 dairy & animal science Heat stress Endocrinology Blood chemistry chemistry Circulatory system Medicine Female Hemoglobin Chickens Heat-Shock Response |
Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) |
ISSN: | 2045-2322 |
DOI: | 10.1038/s41598-021-83293-y |
Popis: | Heat stress (HS) is devastating to the poultry industry due to its adverse effects on animal well-being and performance. The effects of heat stress are typically measured using a portable i-STAT blood analyzer that quantifies circulatory hemoglobin concentration and other blood chemistry parameters. Here, we used diffuse reflectance spectroscopy (DRS) as a novel non-invasive method to directly determine changes in hematological parameters in the breast tissues of live heat-stressed broilers. Three-week-old male broilers were randomly subjected to two environmental conditions (thermoneutral, TN, 24 °C vs. cyclic heat stress, HS, 35 °C, 12 h/day). Optical spectra were acquired using DRS to monitor breast hemoglobin (Hb) concentration and vascular oxygen saturation (sO2) at three time points: at baseline prior to heat stress, 2 days, and 21 days after initiation of HS. While i-STAT did not demonstrate a discernible change due to HS in circulatory hemoglobin, DRS found a significant decrease in breast Hb and sO2 after exposure to chronic HS. The decrease in sO2 was found to be due to a decrease in oxygenated hemoglobin concentration, indicating a large increase in oxygen consumption in heat-stressed broilers. Our results demonstrate that DRS could potentially be used to study the effects of HS directly in specific organs of interest, such as the breast and thigh, to improve meat quality. |
Databáze: | OpenAIRE |
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